Division of Plant Microbe Interaction, Council of Scientific and Industrial Research-National Botanical Research Institute, Lucknow, India
Division of Plant Microbe Interaction, Council of Scientific and Industrial Research-National Botanical Research Institute, Lucknow, India.
Appl Environ Microbiol. 2018 Apr 2;84(8). doi: 10.1128/AEM.02845-17. Print 2018 Apr 15.
Endophytes have been explored and found to perform an important role in plant health. However, their effects on the host physiological function and disease management remain elusive. The present study aimed to assess the potential effects of endophytes, singly as well as in combination, in (L.) Dunal, on various physiological parameters and systemic defense mechanisms against Seeds primed with the endophytic bacteria and individually and in combination demonstrated an enhanced vigor index and germination rate. Interestingly, plants treated with the two-microbe combination showed the lowest plant mortality rate (28%) under stress. Physiological profiling of treated plants showed improved photosynthesis, respiration, transpiration, and stomatal conductance under pathogenic stress. Additionally, these endophytes not only augmented defense enzymes and antioxidant activity in treated plants but also enhanced the expression of salicylic acid- and jasmonic acid-responsive genes in the stressed plants. Reductions in reactive oxygen species (ROS) and reactive nitrogen species (RNS) along with enhanced callose deposition in host plant leaves corroborated well with the above findings. Altogether, the study provides novel insights into the underlying mechanisms behind the tripartite interaction of endophyte-- and underscores their ability to boost plant health under pathogen stress. is well known for producing several medicinally important secondary metabolites. These secondary metabolites are required by various pharmaceutical sectors to produce life-saving drugs. However, the cultivation of faces severe challenge from leaf spot disease caused by To keep pace with the rising demand for this plant and considering its capacity for cultivation under field conditions, the present study was undertaken to develop approaches to enhance production of through intervention using endophytes. Application of bacterial endophytes not only suppresses the pathogenicity of but also mitigates excessive ROS/RNS generation via enhanced physiological processes and antioxidant machinery. Expression profiling of plant defense-related genes further validates the efficacy of bacterial endophytes against leaf spot disease.
内生菌在植物健康中发挥着重要作用，这一作用已被广泛研究和发现。然而，它们对宿主生理功能和疾病管理的影响仍然难以捉摸。本研究旨在评估内生菌（单独或组合）对 （L.）Dunals 的潜在影响，对各种生理参数和系统防御机制进行评估，以应对 胁迫。用内生细菌 和 单独和组合处理种子，表现出活力指数和发芽率的显著提高。有趣的是，在 胁迫下，用两种微生物组合处理的植物死亡率最低（28%）。处理植物的生理特征分析显示，在病原菌胁迫下，光合作用、呼吸作用、蒸腾作用和气孔导度得到改善。此外，这些内生菌不仅增强了处理植物的防御酶和抗氧化活性，还增强了胁迫植物中水杨酸和茉莉酸响应基因的表达。活性氧（ROS）和活性氮（RNS）的减少以及宿主植物叶片中几丁质的沉积增加与上述发现相符。总的来说，该研究提供了内生菌- 三方相互作用背后潜在机制的新见解，并强调了它们在病原体胁迫下增强植物健康的能力。 以产生多种药用重要次生代谢物而闻名。这些次生代谢物是各个制药部门生产救命药物所必需的。然而，由于 引起的叶斑病， 的种植面临严重挑战。为了满足对这种植物的需求增长，并考虑到其在田间条件下的种植能力，本研究旨在开发通过内生菌干预来提高 产量的方法。细菌内生菌的应用不仅抑制了 的致病性，还通过增强生理过程和抗氧化机制减轻了ROS/RNS 的过度产生。植物防御相关基因的表达谱进一步验证了细菌内生菌对叶斑病的疗效。
