Sathiyabama Muthukrishnan, Parthasarathy Ramalingam
a Department of Botany , Bharathidasan University , Tiruchirappalli , India.
Nat Prod Res. 2018 Jul;32(13):1573-1577. doi: 10.1080/14786419.2017.1389934. Epub 2017 Oct 16.
Withanolides, the secondary metabolite from Withania species is used in Ayurvedic medicine and now proved to have potential use in treating cardiovascular, Alzheimer's disease, etc. Its production in plants varies between genotypes with very low yield. For improved industrial commercialisation, there is a need to increase its production. Endophytic fungi are symbiotically associated with plants and can synthesise the same bioactive compounds and natural products as their host plant. There are no reports available on withanolide-producing (endophytic) fungi. The present study identified an endophytic fungus (Taleromyces pinophilus) from leaves of Withania somnifera which produces withanolides in the medium. The structure of withanolide was confirmed by H NMR, LC-MS analyses and quantified by HPLC analysis. The fungus produces high amount of withanolide when compared to leaf and root of W. somnifera. The fungus can be exploited to produce the withanolide to meet its demand.
睡茄内酯是睡茄属植物的次生代谢产物，用于阿育吠陀医学，目前已被证明在治疗心血管疾病、阿尔茨海默病等方面具有潜在用途。其在植物中的产量因基因型而异，产量极低。为了促进工业商业化，有必要提高其产量。内生真菌与植物共生，能够合成与其宿主植物相同的生物活性化合物和天然产物。目前尚无关于产生睡茄内酯的（内生）真菌的报道。本研究从印度人参的叶片中鉴定出一种内生真菌（嗜松泰勒菌），该真菌可在培养基中产生睡茄内酯。通过核磁共振氢谱（H NMR）、液相色谱 - 质谱联用（LC - MS）分析确认了睡茄内酯的结构，并通过高效液相色谱（HPLC）分析进行了定量。与印度人参的叶片和根相比，该真菌能产生大量的睡茄内酯。这种真菌可用于生产睡茄内酯以满足其需求。
