Research Laboratories for Health Science & Food Technologies, Kirin Company, Limited, Yokohama, Kanagawa, Japan.
Central Laboratories for Key Technologies, Kirin Company, Limited, Yokohama, Kanagawa, Japan.
Sci Rep. 2018 Feb 16;8(1):3147. doi: 10.1038/s41598-018-21527-2.
Plasmacytoid dendritic cells (pDCs) play a key role in the immune response against viruses. In addition, recent research has suggested that pDCs possess direct and indirect tumoricidal activities. We previously found that a lactic acid bacteria strain, Lactococcus lactis JCM 5805 (LC-Plasma), stimulated pDCs and prevented viral infection in mouse and human studies. Meanwhile, emulsifiers have recently been highlighted as candidate adjuvants for some viral vaccines and cancer immunotherapies. In this study, we discovered some specific emulsifiers, mainly consisting of sucrose fatty acid esters, that drastically enhance the potency of LC-Plasma to activate pDCs in vitro. The emulsifiers promoted the efficient uptake of LC-Plasma by pDCs and the ratio of pDCs that took up LC-Plasma correlated with the activity of pDCs. In addition, an in vivo study showed that oral treatment with LC-Plasma mixed with an emulsifier induced a higher expression of genes related to anti-viral immunity in the lung compared to treatment with LC-Plasma alone. Both LC-Plasma and the emulsifiers used in this study have been confirmed to be safe for human use. Therefore, LC-Plasma mixed with an emulsifier might be a useful tool for certain anti-cancer and anti-viral therapies.
浆细胞样树突状细胞 (pDCs) 在抗病毒免疫反应中发挥关键作用。此外，最近的研究表明，pDCs 具有直接和间接的杀肿瘤活性。我们之前发现一种乳酸菌菌株，乳球菌 lactis JCM 5805（LC-Plasma），在小鼠和人类研究中刺激 pDCs 并预防病毒感染。同时，乳化剂最近被强调为一些病毒疫苗和癌症免疫疗法的候选佐剂。在这项研究中，我们发现了一些特定的乳化剂，主要由蔗糖脂肪酸酯组成，可显著增强 LC-Plasma 在体外激活 pDCs 的效力。乳化剂促进了 pDCs 对 LC-Plasma 的有效摄取，摄取 LC-Plasma 的 pDCs 比例与 pDCs 的活性相关。此外，体内研究表明，与单独使用 LC-Plasma 相比，口服用 LC-Plasma 与乳化剂混合处理可诱导肺中与抗病毒免疫相关的基因更高表达。本研究中使用的 LC-Plasma 和乳化剂均已被证实对人体安全。因此，LC-Plasma 与乳化剂混合可能是某些抗癌和抗病毒疗法的有用工具。
