Bernardino Raquel L, Alves Marco G, Oliveira Pedro F
Unit for Multidisciplinary Research in Biomedicine (UMIB), Laboratory of Cell Biology, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
Methods Mol Biol. 2018;1748:1-8. doi: 10.1007/978-1-4939-7698-0_1.
The successful isolation and culture of Sertoli cells depend on a series of delicate processes of mechanical isolation and enzymatic digestion of the testicular tissue, taking advantage of an array of enzymes (such as DNAse, collagenase, and pancreatin) in order to digest the extracellular matrix components. The complexity of these processes may present some differences depending on the origin of the testicular sample (whole tissue or biopsy) and of the species in question. Rat and mouse Sertoli cells are obtained by a similar protocol, whereas bovine and human Sertoli cells require a more extensive mechanical and enzymatic processing.
支持细胞的成功分离和培养依赖于一系列精细的睾丸组织机械分离和酶消化过程，利用多种酶（如脱氧核糖核酸酶、胶原酶和胰蛋白酶）来消化细胞外基质成分。这些过程的复杂性可能因睾丸样本的来源（全组织或活检）以及所涉及的物种而存在一些差异。大鼠和小鼠的支持细胞通过类似的方案获得，而牛和人的支持细胞则需要更广泛的机械和酶处理。
