Laboratório de Genética Molecular de Fungos (LGMF)/BIOAGRO, Universidade Federal de Viçosa, Viçosa, Minas Gerais, CEP: 36570-900, Brazil.
Núcleo de Análise de Biomoléculas (NuBioMol), Centro de Ciências Biológicas, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Appl Microbiol Biotechnol. 2018 Mar;102(6):2763-2778. doi: 10.1007/s00253-018-8812-0. Epub 2018 Feb 16.
Fungi of the genus Colletotrichum are economically important and are used as models in plant-pathogen interaction studies. In this study, the complete mitochondrial genomes of two Colletotrichum lindemuthianum isolates were sequenced and compared with the mitochondrial genomes of seven species of Colletotrichum. The mitochondrial genome of C. lindemuthianum is a typical circular molecule 37,446 bp (isolate 89 A 2-3) and 37,440 bp (isolate 83.501) in length. The difference of six nucleotides between the two genomes is the result of a deletion in the ribosomal protein S3 (rps3) gene in the 83.501 isolate. In addition, substitution of adenine for guanine within the rps3 gene in the mitochondrial genome of the 83.501 isolate was observed. Compared to the previously sequenced C. lindemuthianum mitochondrial genome, an exon no annotated in the cytochrome c oxidase I (cox1) gene and a non-conserved open reading frame (ncORF) were observed. The size of the mitochondrial genomes of the seven species of Colletotrichum was highly variable, being attributed mainly to the ncORF, ranging from one to 10 and also from introns ranging from one to 11 and which encode a total of up to nine homing endonucleases. This paper reports for the first time by means of transcriptome that then ncORFs are transcribed in Colletotrichum spp. Phylogeny data revealed that core mitochondrial genes could be used as an alternative in phylogenetic relationship studies in Colletotrichum spp. This work contributes to the genetic and biological knowledge of Colletotrichum spp., which is of great economic and scientific importance.
炭疽菌属真菌具有重要的经济意义，并且被用作植物-病原体相互作用研究的模型。在这项研究中，对两个炭疽菌 Lindemuthianum 分离株的完整线粒体基因组进行了测序，并与七种炭疽菌的线粒体基因组进行了比较。炭疽菌 Lindemuthianum 的线粒体基因组是一个典型的圆形分子，长度为 37446bp（分离株 89 A 2-3）和 37440bp（分离株 83.501）。这两个基因组之间的六个核苷酸差异是核糖体蛋白 S3（rps3）基因在 83.501 分离株中缺失的结果。此外，在 83.501 分离株的线粒体基因组中，rps3 基因内腺嘌呤取代了鸟嘌呤。与以前测序的炭疽菌 Lindemuthianum 线粒体基因组相比，在细胞色素 c 氧化酶 I（cox1）基因中观察到一个未注释的外显子，并且观察到一个非保守的开放阅读框（ncORF）。七种炭疽菌的线粒体基因组大小高度可变，主要归因于 ncORF，范围从一个到十个，也归因于从一个到十一个的内含子，总共编码多达九个归巢内切酶。本文首次通过转录组报告了 ncORFs 在炭疽菌属中被转录。系统发育数据表明，核心线粒体基因可作为炭疽菌属系统发育关系研究的替代方法。这项工作为炭疽菌属的遗传和生物学知识做出了贡献，具有重要的经济和科学意义。
