Nie Yong, Zhao Heng, Wang Zimin, Zhou Zhengyu, Liu Xiaoyong, Huang Bo
Anhui Provincial Key Laboratory for Microbial Pest Control, Anhui Agricultural University, Hefei, China.
School of Civil Engineering and Architecture, Anhui University of Technology, Ma'anshan, China.
Front Microbiol. 2021 Nov 11;12:765733. doi: 10.3389/fmicb.2021.765733. eCollection 2021.
The genus s.s. was newly delimited from s.l. In order to gain insight into its mitochondrial genetic background, this study sequenced six mitochondrial genomes of the genus s.s. These mitogenomes were all composed of circular DNA molecules, ranging from 29,253 to 48,417 bp in size and from 26.61 to 27.90% in GC content. The order and direction for 14 core protein-coding genes (PCGs) were identical, except for the gene lost in , , and , and rearranged in the other s.s. species. Besides, the gene split the gene in . Phylogenomic analysis based on the 14 core PCGs confirmed that all s.s. species formed a monophyly in the lineage. The number and length of introns were the main factors contributing to mitogenomic size, and deep variations and potential transfer were detected in introns. In addition, gene transfer occurred between the mitochondrial and nuclear genomes. This study promoted the understanding of the evolution and phylogeny of the s.s. genus.
狭义属是从广义属中新划分出来的。为了深入了解其线粒体遗传背景，本研究对狭义属的六个线粒体基因组进行了测序。这些线粒体基因组均由环状DNA分子组成，大小在29,253至48,417 bp之间，GC含量在26.61%至27.90%之间。14个核心蛋白质编码基因（PCGs）的顺序和方向相同，除了在[具体物种1]、[具体物种2]和[具体物种3]中丢失的[基因名称]基因，以及在其他狭义属物种中发生重排的该基因。此外，[基因名称]基因在[具体物种4]中分裂了[另一基因名称]基因。基于14个核心PCGs的系统基因组分析证实，所有狭义属物种在[谱系名称]谱系中形成一个单系群。内含子的数量和长度是导致线粒体基因组大小差异的主要因素，并且在内含子中检测到深度变异和潜在转移。此外，线粒体基因组与核基因组之间发生了基因转移。本研究促进了对狭义属进化和系统发育的理解。
