College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
J Pineal Res. 2018 Aug;65(1):e12477. doi: 10.1111/jpi.12477. Epub 2018 Mar 25.
Deoxynivalenol (DON) is one of the most prevalent fusarium mycotoxins in feedstuff and food. DON causes detrimental effects on human and animal reproductive systems by inducing oxidative stress and apoptosis. However, melatonin is a multifunctional endogenous hormone that plays crucial roles in the development of animal germ cells and embryos as a robust deoxidizer. In this study, we explored the effects of melatonin on the DON exposure mouse oocytes. Our in vitro and in vivo results showed that DON adversely affected mouse oocyte maturation and early embryo cleavage, while melatonin administration ameliorated the toxic effects of DON. DON exposure disrupted the meiotic spindle formation and kinetochore-microtubule attachment, which induced aneuploidy in oocytes. This might be through DON effects on the acetylated tubulin level. Moreover, we found that DON exposure caused the alteration of DNA and histone methylation level, which might affect early embryo cleavage. The toxic effects of DON on oocytes might be through its induction of oxidative stress-mediated early apoptosis, while the treatment with melatonin significantly ameliorated these phenotypes in DON-exposed mouse oocytes. Collectively, our results indicated the protection effects of melatonin against defects induced by DON during mouse oocyte meiotic maturation.
脱氧雪腐镰刀菌烯醇（DON）是饲料和食品中最常见的镰刀菌毒素之一。DON 通过诱导氧化应激和细胞凋亡，对人类和动物的生殖系统造成有害影响。然而，褪黑素是一种多功能的内源性激素，作为一种强大的抗氧化剂，在动物生殖细胞和胚胎的发育中起着至关重要的作用。在这项研究中，我们探讨了褪黑素对 DON 暴露的小鼠卵母细胞的影响。我们的体外和体内结果表明，DON 对小鼠卵母细胞成熟和早期胚胎卵裂有不良影响，而褪黑素的给药改善了 DON 的毒性作用。DON 暴露破坏了减数分裂纺锤体的形成和着丝粒微管的附着，导致卵母细胞非整倍体。这可能是通过 DON 对乙酰化微管蛋白水平的影响。此外，我们发现 DON 暴露导致 DNA 和组蛋白甲基化水平的改变，这可能会影响早期胚胎卵裂。DON 对卵母细胞的毒性作用可能是通过其诱导的氧化应激介导的早期细胞凋亡，而褪黑素的处理显著改善了 DON 暴露的小鼠卵母细胞中的这些表型。总的来说，我们的研究结果表明，褪黑素对 DON 在小鼠卵母细胞减数成熟过程中诱导的缺陷具有保护作用。
