Graduate Institute of Chinese Medicine, China Medical University, Taichung, Taiwan; School of Chinese Medicine, China Medical University, Taichung, Taiwan.
Graduate Institute of Chinese Medicine, China Medical University, Taichung, Taiwan; School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Medical Research, China Medical University Hospital, Taichung, Taiwan.
Colloids Surf B Biointerfaces. 2018 May 1;165:67-73. doi: 10.1016/j.colsurfb.2018.02.013. Epub 2018 Feb 10.
Zicao is a traditional Chinese herbal medicine that has been used for the topical treatment of wounds in the form of oil-based ointment for several hundred years. To overcome the disadvantages of oil-based ointment such as irritation, discomfort, and difficulty in cleaning, this study developed a water-based topical formulation of Zicao. An ethanol extract of Zicao was included in 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) to form a water-soluble Zicao-HP-β-CD complex. The formation of the Zicao-HP-β-CD complex was determined using LC-MS, H NMR, ROSEY, and solubility analysis. The bioactivity of Zicao-HP-β-CD complex in aqueous solution was evaluated using cellular uptake in vitro and experimental excision wounds in vivo. The LC-MS, H NMR, ROESY, and solubility analyses results show that Zicao extract was successfully included by the HP-β-CD. The results of the cellular uptake in vitro and wound healing in vivo suggest that the effect of Zicao was enhanced following the formation of the Zicao-HP-β-CD complex. Therefore, we concluded that complexation with HP-β-CD might provide a potential method for developing an effective water-based topical solution of Zicao.
紫草是一种传统的中草药，几百年来一直以油膏的形式局部用于治疗伤口。为了克服油膏的缺点，如刺激性、不适感和清洁困难，本研究开发了一种紫草的水基局部制剂。紫草的乙醇提取物被包含在 2-羟丙基-β-环糊精（HP-β-CD）中，形成水溶性的紫草-HP-β-CD 配合物。LC-MS、1H NMR、ROESY 和溶解度分析用于确定紫草-HP-β-CD 配合物的形成。紫草-HP-β-CD 配合物在水溶液中的生物活性通过体外细胞摄取和体内实验性切除伤口进行评估。LC-MS、1H NMR、ROESY 和溶解度分析结果表明，HP-β-CD 成功地包含了紫草提取物。体外细胞摄取和体内伤口愈合的结果表明，形成紫草-HP-β-CD 配合物后，紫草的效果得到了增强。因此，我们得出结论，与 HP-β-CD 的络合可能为开发有效的紫草水基局部溶液提供一种潜在的方法。
