Guo Lei, Cao Xi, Yang Shulin, Wang Xintong, Wen Ying, Zhang Fei, Chen Hui, Wang Le
1Jiangsu Key Laboratory of Marine Bioresources and Environment, Huaihai Institute of Technology, Lianyungang, 222005 China.
2Jiangsu Key Laboratory of Marine Biotechnology, Co-innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005 China.
3 Biotech. 2019 Apr;9(4):123. doi: 10.1007/s13205-019-1663-2. Epub 2019 Mar 5.
To increase the water solubility of questin and broaden its application in preventing and treating diseases in aquaculture, an inclusion complex of questin with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared by stirring and coevaporation strategy. The results of thin-layer chromatography and nuclear magnetic resonance spectrum confirmed the inclusion of questin into HP-β-CD. The aqueous solubility of questin in the inclusion complex reached (62.63 ± 1.21) µg/mL, which was 110 times of questin's original solubility. The preliminary agar diffusion method indicated that questin-HP-β-CD inclusion complex showed enhanced antibacterial activity against compared with free questin. This finding provided a reliable basis for the further application of questin as aquatic antibacterial agent.
为提高喹赛因的水溶性并拓宽其在水产养殖疾病防治中的应用，采用搅拌和共蒸发法制备了喹赛因与2-羟丙基-β-环糊精（HP-β-CD）的包合物。薄层色谱和核磁共振谱结果证实喹赛因被包合进HP-β-CD中。喹赛因在包合物中的水溶性达到(62.63 ± 1.21) μg/mL，是喹赛因原溶解度的110倍。初步琼脂扩散法表明，与游离喹赛因相比，喹赛因-HP-β-CD包合物对[此处原文缺失比较对象]显示出增强的抗菌活性。这一发现为喹赛因作为水产抗菌剂的进一步应用提供了可靠依据。
