Universidade Tiradentes, Av. Murilo Dantas, 300, Farolânida, Aracaju, SE, Brazil.
Universidade Tiradentes, Av. Murilo Dantas, 300, Farolânida, Aracaju, SE, Brazil.
J Photochem Photobiol B. 2018 Mar;180:198-207. doi: 10.1016/j.jphotobiol.2018.01.028. Epub 2018 Jan 31.
The negative effects triggered by ultraviolet radiation, such as premature aging and carcinogenesis, have motivated several studies on photoprotection. Recent strategies for photoprotection have included the incorporation of natural antioxidant and anti-inflammatory compounds, such as flavonoids, into sunscreens and the oral administration of natural antioxidant extracts. Brazilian Red propolis extract contains isoflavonoids with antioxidant and anti-inflammatory activities. Here, we investigate the photoprotective effects of orally- or topically-administered formulations containing hydroalcoholic extract of red propolis (HERP) in a rodent model. HERP showed markers identified as: daidzein (4.68 μg/mL), formononetin (31.81 μg/mL) and biochanin A (9.58 μg/mL). A fourth peak was found in the chromatogram but was not identified. The antioxidant activity of HERP was calculated to be 3.07 mmol Trolox/g and 2.13 mmol Trolox/g, respectively. Topical HERP exerted a protective action against UVB radiation, which was similar to that exerted by oxybenzone filter. Oral HERP as an adjuvant treatment did not increase sunburn protection. However, the oral administration of HERP presented chemoprotective and anti-inflammatory activity (p < 0.05) similar or better than Polypodium leucotomos oral treatment (positive control). In conclusion, topical administration of HERP has photoprotective activity in a murine model and the mechanisms of protection can be related to the antioxidant and anti-inflammatory characteristics of HERP compounds.
紫外线辐射引发的负面效应，如皮肤过早衰老和致癌作用，激发了许多关于光保护的研究。最近的光保护策略包括将天然抗氧化剂和抗炎化合物，如类黄酮，纳入防晒霜和口服天然抗氧化提取物中。巴西红蜂胶提取物含有具有抗氧化和抗炎活性的异黄酮。在这里，我们研究了含蜂胶水醇提取物（HERP）的口服或局部制剂在啮齿动物模型中的光保护作用。HERP 显示出以下被识别的标志物：大豆苷（4.68μg/mL）、芒柄花素（31.81μg/mL）和染料木素 A（9.58μg/mL）。在色谱图中发现了第四个峰，但未被识别。HERP 的抗氧化活性分别计算为 3.07mmol Trolox/g 和 2.13mmol Trolox/g。局部应用 HERP 对 UVB 辐射具有保护作用，与羟苯酮滤光剂的作用相似。口服 HERP 作为辅助治疗并没有增加晒伤保护。然而，口服 HERP 表现出化学保护和抗炎活性（p<0.05），与 Polypodium leucotomos 口服治疗（阳性对照）相似或更好。总之，局部应用 HERP 在小鼠模型中具有光保护活性，保护机制可能与 HERP 化合物的抗氧化和抗炎特性有关。
