Leng Xue, Liu Dongxu, Li Jianming, Shi Kun, Zeng Fanli, Zong Ying, Liu Yi, Sun Zhibo, Zhang Shanshan, Liu Yadong, Du Rui
College of Chinese Medicine Material, Jilin Agricultural University, No. 2888, Xincheng Street, Changchun, 130118, People's Republic of China.
College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, 132101, People's Republic of China.
Arch Virol. 2018 May;163(5):1241-1251. doi: 10.1007/s00705-018-3754-5. Epub 2018 Feb 17.
Aleutian mink disease is the most important disease in the mink-farming industry worldwide. So far, few large-scale molecular epidemiological studies of AMDV, based on the NS1 and VP2 genes, have been conducted in China. Here, eight new Chinese isolates of AMDV from three provinces in north-east China were analyzed to clarify the molecular epidemiology of AMDV. The seroprevalence of AMDV in north-east China was 41.8% according to counterimmuno-electrophoresis. Genetic variation analysis of the eight isolates showed significant non-synonymous substitutions in the NS1 and VP2 genes, especially in the NS1 gene. All eight isolates included the caspase-recognition sequence NS1:285 (DQTD↓S), but not the caspase recognition sequence NS1:227 (INTD↓S). The LN1 and LN2 strains had a new 10-amino-acid deletion in-between amino acids 28-37, while the JL3 strain had a one-amino-acid deletion at position 28 in the VP2 protein, compared with the AMDV-G strain. Phylogenetic analysis based on most of NS1 (1755 bp) and complete VP2 showed that the AMDV genotypes did not cluster according to their pathogenicity or geographic origin. Local and imported ADMV species are all prevalent in mink-farming populations in the north-east of China. This is the first study to report the molecular epidemiology of AMDV in north-east China based on most of NS1 and the complete VP2, and further provides information about polyG deletions and new variations in the amino acid sequences of NS1 and VP2 proteins. This report is a good foundation for further study of AMDV in China.
阿留申水貂病是全球水貂养殖业中最重要的疾病。到目前为止，中国很少有基于NS1和VP2基因对阿留申水貂病病毒（AMDV）进行的大规模分子流行病学研究。在此，对来自中国东北地区三个省份的8株新的AMDV中国分离株进行分析，以阐明AMDV的分子流行病学。根据对流免疫电泳，中国东北地区AMDV的血清阳性率为41.8%。对这8株分离株的遗传变异分析显示，NS1和VP2基因存在显著的非同义替换，尤其是在NS1基因中。所有8株分离株都包含半胱天冬酶识别序列NS1:285（DQTD↓S），但不包含半胱天冬酶识别序列NS1:227（INTD↓S）。与AMDV-G株相比，LN1和LN2株在氨基酸28 - 37之间有一个新的10个氨基酸的缺失，而JL3株在VP2蛋白的第28位有一个氨基酸缺失。基于大部分NS1（1755 bp）和完整VP2的系统发育分析表明，AMDV基因型并未根据其致病性或地理来源聚类。本地和进口的ADMV毒株在中国东北地区的水貂养殖群体中均普遍存在。这是首次基于大部分NS1和完整VP2报道中国东北地区AMDV的分子流行病学，并进一步提供了关于NS1和VP2蛋白氨基酸序列中多聚G缺失和新变异的信息。本报告为中国进一步研究AMDV奠定了良好基础。
