Gutkowska J, Bonan R, Roy D, Bourassa M, Garcia R, Thibault G, Genest J, Cantin M
Biochem Biophys Res Commun. 1986 Aug 29;139(1):287-95. doi: 10.1016/s0006-291x(86)80111-5.
A reproducible and sensitive radioimmunoassay (RIA) was developed to measure ANF in human plasma. Immunoreactive ANF was extracted from plasma with Sep-Pak cartridges, using 0.2% ammonium acetate (pH 4) with acetonitrile. The sensitivity of the assay was 3.9 pg/ml. The coefficient of variance for inter-assay and intra-assay was 16.8% and 6.8%, respectively. In normal healthy subjects (n = 67), ANF content was 11.9 +/- 1.3 pg/ml (mean +/- SEM). Significantly-higher ANF concentrations were found in proximal coronary sinus blood, being 6 to 37 times greater than in the peripheral circulation. Comparison of the prior extraction method with direct RIA revealed a good correlation (r = 91) in samples containing higher than 100 pg/ml ANF. No correlation was observed with lower values. The elution profiles of reverse-phase HPLC of peripheral and coronary sinus plasma extracts were similar but somewhat complex, with the main immunoreactive peak corresponding to a low-molecular-weight peptide.
开发了一种可重复且灵敏的放射免疫分析法（RIA）来测定人血浆中的心钠素（ANF）。使用含乙腈的0.2%醋酸铵（pH 4），通过Sep-Pak柱从血浆中提取免疫反应性心钠素。该分析方法的灵敏度为3.9 pg/ml。批间和批内变异系数分别为16.8%和6.8%。在正常健康受试者（n = 67）中，心钠素含量为11.9 +/- 1.3 pg/ml（平均值 +/- 标准误）。在近端冠状窦血中发现心钠素浓度显著更高，比外周循环中的浓度高6至37倍。将先前的提取方法与直接放射免疫分析法进行比较，发现在心钠素含量高于100 pg/ml的样本中具有良好的相关性（r = 91）。对于较低的值未观察到相关性。外周和冠状窦血浆提取物的反相高效液相色谱洗脱图谱相似但有些复杂，主要免疫反应峰对应一种低分子量肽。
