School of Chemical Engineering, Institute of Pharmaceutical Engineering Technology and Application, Key Laboratory of Green Chemistry of Sichuan Institutes of Higher Education, Sichuan University of Science & Engineering, Zigong 643000, China.
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Chin J Nat Med. 2018 Feb;16(2):139-142. doi: 10.1016/S1875-5364(18)30040-2.
The present study was designed to determine the chemical constituents of the stem tuber of Pinellia pedatisecta. The chemical constituents were isolated and purified by various chromatographic techniques, and their structures were elucidated on the basis of physicochemical properties and spectral data. Three new alkaloids (compounds 1, 2, and 3) were obtained and identified as 9-((5-methoxypyridin-2-yl)methyl)-9H-purin-6-amine (1), 4-(2-(2, 5-dioxopyrrolidin-1-yl)ethyl)phenyl acetate (2), and N-(9-((5-methoxypyridin-2-yl)methyl)-9H-purin-6-yl)acetamide (3). These compounds were evaluated for their cytotoxicity against human cervical cancer HeLa cells. Compounds 1 and 3 significantly inhibited the proliferation of HeLa cells with IC values being 3.02 ± 0.54 and 7.16 ± 0.62 μmol·L, respectively.
本研究旨在确定半夏块茎的化学成分。通过各种色谱技术对化学成分进行分离和纯化，并根据理化性质和光谱数据阐明其结构。得到了三个新的生物碱（化合物 1、2 和 3），并鉴定为 9-（（5-甲氧基吡啶-2-基）甲基）-9H-嘌呤-6-胺（1）、4-（2-（2,5-二氧代吡咯烷-1-基）乙基）苯乙酸酯（2）和 N-（9-（（5-甲氧基吡啶-2-基）甲基）-9H-嘌呤-6-基）乙酰胺（3）。这些化合物被评估对人宫颈癌 HeLa 细胞的细胞毒性。化合物 1 和 3 显著抑制 HeLa 细胞的增殖，IC 值分别为 3.02±0.54 和 7.16±0.62 μmol·L。
