School of Marine Science & Technology, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
Carbohydr Polym. 2018 Apr 15;186:17-24. doi: 10.1016/j.carbpol.2018.01.036. Epub 2018 Jan 11.
A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study. Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system. RPM could flocculate marine microalgae Chlorella salina with a FR of 74.1%. The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content. The molecular weights of RPMP-1 and RPMP-2 were 5.7 kDa and 18.0 kDa, respectively. It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation.
本研究首次报道了一种新型天然生物絮凝剂资源——菲律宾蛤仔粘连泥（RPM），其中含有有效的絮凝成分。实验结果表明，在去离子水和海水实验体系中，RPM 对高岭土的最大絮凝活性（FR）分别达到 86.7%和 91.8%。RPM 对海洋微藻盐藻的 FR 为 74.1%。粗 RPM 多糖提取物（RPMP）由 97.8%（w/w）多糖和 2.2%（w/w）蛋白质组成，并且首次发现纯 RPMP 的功能成分是两种复杂的杂多糖 RPMP-1 和 RPMP-2，它们的单糖组成除葡萄糖含量外基本相同。RPMP-1 和 RPMP-2 的分子量分别为 5.7 kDa 和 18.0 kDa。有趣的是，发现 RPMP-1 和 RPMP-2 在质量比为 2:1 时表现出协同絮凝活性为 65.6%，这表明原始比例对协同絮凝作用具有重要意义。
