School of Marine Science & Technology, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
School of Marine Science & Technology, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
Bioresour Technol. 2019 Jun;281:480-484. doi: 10.1016/j.biortech.2019.02.080. Epub 2019 Mar 7.
In this study a preparation strategy was attempted to produce a substitute (BBF) for the Ruditapes philippinarum conglutination mud (RPM), which was newly discovered to be a promising natural bioflocculant resource. A stable yield of 73.77 ± 1.79 g L BBF was established by a sequential batch fermentation under optimized conditions via single factor experiments. BBF attained similar flocculation performance as RPM, showing a maximum flocculation rate of 87.92 ± 0.65%. BBF had significant decolorization efficiency on methylene blue, crystal violet and malachite green by 98.78 ± 0.46%, 89.37 ± 0.35% and 99.11 ± 0.17%, respectively. BBF could harvest microalgae Chlorella salina by 84.38 ± 0.57%. High throughput sequencing revealed that Vibrio and Bacillus might be the extracellular polysaccharides producers. The successful preparation will enable a potential industrial production of BBF thus avoid scattered collection of RPM.
在这项研究中，尝试了一种制备策略，以生产一种新发现的有前途的天然生物絮凝剂资源——菲律宾蛤仔凝结泥浆（RPM）的替代品（BBF）。通过单因素实验优化条件下的分批发酵，成功建立了稳定的产量为 73.77±1.79 g·L-1 的 BBF。BBF 表现出与 RPM 相似的絮凝性能，最大絮凝率为 87.92±0.65%。BBF 对亚甲基蓝、结晶紫和孔雀石绿具有显著的脱色效率，分别达到 98.78±0.46%、89.37±0.35%和 99.11±0.17%。BBF 可以通过 84.38±0.57%的方法收获小球藻 Chlorella salina。高通量测序显示，弧菌和芽孢杆菌可能是胞外多糖的产生菌。成功的制备将使 BBF 能够进行潜在的工业化生产，从而避免 RPM 的分散收集。
