从凝集泥天然生物絮凝剂中分离出的菌株所产生的胞外多糖的特性
Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of conglutination mud.
作者信息
Feng Lijuan, Qian Tingting, Yang Guangfeng, Mu Jun
机构信息
Zhejiang Provincial Key Laboratory of Petrochemical Pollution Control, Zhejiang Ocean University, Zhoushan, Zhejiang, China.
School of Ecology and Environment, Hainan Tropical Ocean University, Sanya, Hainan, China.
出版信息
Front Microbiol. 2023 Jan 11;13:1068922. doi: 10.3389/fmicb.2022.1068922. eCollection 2022.
Three novel types of exopolysaccharides (EPS) EPS-S8, EPS-S5, and EPS-F10 were extracted and purified from bacterial isolates sp. GHS8, sp. GHS5 and sp. GHF10, which were originated from natural bioflocculant of conglutination mud (RPM), respectively. The EPS had similar function groups C-H, N-H, C-O, and C = O. The EPS were composed of different monosaccharides (EPS-F10, Man: GlcN: GlcUA: GalUA = 1:0.66:5.75:0.51; EPS-S5, Man: Gal: GlcN: Rib = 1: 0.50: 2.94: 0.26; EPS-S8, Man: Gal: GlcN = 1:1.54:7.69). The molecular weights (Mw) of EPS were ordered as 51.4 kDa (EPS-S5) > 9.15 kDa (EPS-S8) > 4.41 kDa (EPS-F10). Three types of EPS all showed higher peak flocculation activities than the reported crude EPS from the RPM. Besides, the EPS also exhibited efficient decoloration and antioxidation activities, especially for EPS-S8, which might be due to the low Mw and specific monosaccharide composition.
从分别源自团聚泥（RPM）天然生物絮凝剂的细菌分离株sp. GHS8、sp. GHS5和sp. GHF10中提取并纯化出三种新型胞外多糖（EPS），即EPS-S8、EPS-S5和EPS-F10。这些EPS具有相似的官能团C-H、N-H、C-O和C=O。EPS由不同的单糖组成（EPS-F10，甘露糖：葡糖胺：葡萄糖醛酸：半乳糖醛酸 = 1:0.66:5.75:0.51；EPS-S5，甘露糖：半乳糖：葡糖胺：核糖 = 1: 0.50: 2.94: 0.26；EPS-S8，甘露糖：半乳糖：葡糖胺 = 1:1.54:7.69）。EPS的分子量（Mw）顺序为51.4 kDa（EPS-S5）> 9.15 kDa（EPS-S8）> 4.41 kDa（EPS-F10）。三种类型的EPS均表现出比报道的来自RPM的粗EPS更高的峰值絮凝活性。此外，这些EPS还表现出高效的脱色和抗氧化活性，尤其是EPS-S8，这可能归因于其低分子量和特定的单糖组成。
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