Peiyuan He, Zhiping Hou, Chengjun Song, Chunqing Wang, Bingqing Li, Imam Mustapha Umar
Department of Gastroenterology, The Affiliated Hospital of Chengde Medical University, Chengde, Hebei 067000, China.
Department of Pathology, Chengde Medical University, Chengde, Hebei 067000, China.
Evid Based Complement Alternat Med. 2017;2017:4287890. doi: 10.1155/2017/4287890. Epub 2017 Dec 31.
The aim of this study was to investigate the hepatoprotective effects of resveratrol in alcoholic liver disease (ALD). Alcohol was administered to healthy female rats starting from 6% (v/v) and gradually increased to 20% (v/v) by the fifth week. After 16 weeks of intervention, liver enzymes (aspartate aminotransferase [AST] and alanine aminotransferase [ALT]) were analyzed using a chemistry analyzer, while hepatic antioxidant enzymes, oxidative stress markers, and caspase 3 activity were assessed using ELISA kits. Furthermore, hepatic CYP2E1 protein levels and mRNA levels of antioxidant and inflammation-related genes were determined using western blotting and RT-PCR, respectively. The results showed that resveratrol significantly attenuated alcohol-induced elevation of liver enzymes and improved hepatic antioxidant enzymes. Resveratrol also attenuated alcohol-induced CYP2E1 increase, oxidative stress, and apoptosis (caspase 3 activity). Moreover, genes associated with oxidative stress and inflammation were regulated by resveratrol supplementation. Taken together, the results suggested that resveratrol alleviated ALD through regulation of oxidative stress, apoptosis, and inflammation, which was mediated at the transcriptional level. The data suggests that resveratrol is a promising natural therapeutic agent against chronic ALD.
本研究旨在探讨白藜芦醇对酒精性肝病（ALD）的肝脏保护作用。从第1周开始给健康雌性大鼠灌胃酒精，起始浓度为6%（体积/体积），至第5周逐渐增至20%（体积/体积）。干预16周后，使用化学分析仪分析肝酶（天冬氨酸转氨酶[AST]和丙氨酸转氨酶[ALT]），同时使用酶联免疫吸附测定试剂盒评估肝脏抗氧化酶、氧化应激标志物和半胱天冬酶3活性。此外，分别使用蛋白质印迹法和逆转录-聚合酶链反应测定肝脏CYP2E1蛋白水平以及抗氧化和炎症相关基因的mRNA水平。结果显示，白藜芦醇显著减轻了酒精诱导的肝酶升高，并改善了肝脏抗氧化酶。白藜芦醇还减轻了酒精诱导的CYP2E1增加、氧化应激和细胞凋亡（半胱天冬酶3活性）。此外，补充白藜芦醇可调节与氧化应激和炎症相关的基因。综上所述，结果表明白藜芦醇通过调节氧化应激、细胞凋亡和炎症来减轻酒精性肝病，这一过程在转录水平介导。数据表明白藜芦醇是一种有前景的抗慢性酒精性肝病的天然治疗剂。
