Janarthanam Vishnu Adith, Rajan Panneer Selvam Sundar, Panda Siva Prasad, Panigrahy Uttam Prasad, Gupta Rupesh, Guru Ajay, Issac Praveen Kumar
Department of Medical Biotechnology, Saveetha School of Engineering, Institute of Biotechnology, Saveetha Institute of Medical and Technical Sciences, Thandalam, Chennai, Tamil Nadu, 602105, India.
Department of Chemical Engineering, Saveetha Engineering College, Thandalam, Chennai, Tamil Nadu, 602 105, India.
Mol Biotechnol. 2025 Sep 4. doi: 10.1007/s12033-025-01502-9.
Alcoholic liver disorder (ALD) is one of the most prevalent hepatic ailments worldwide, with oxidative stress and inflammation playing a vital role in disease progression. The current study intended to assess the anti-inflammatory nature of Hamamelitannin (HAM), a gallotannin from Hamamelis virginiana barks, which was predicted to possess anti-inflammatory properties based on in-silico docking analysis. To further explore its effects, we examined the therapeutic effect of HAM against ethanol-mediated inflammation using an in-vivo zebrafish larvae model. Ethanol exposure led to liver inflammation, oxidative stress, lipid accumulation, and hepatocyte apoptosis. However, our findings demonstrated that co-treatment with HAM significantly normalized the larvae's antioxidant enzymes such as SOD (35.81 U/mg protein), CAT (33.83 μ mol/mg protein) and GPx (33.35 U/mg Protein), nitric oxide (NO), lipid accumulation, reactive oxygen species (19.9%), cell death (15.43%), LPO (17.4%), and macrophage infiltration. A gene expression analysis was performed to gain deeper insights into ethanol-induced hepatotoxicity and the protective role of HAM. The results revealed that ethanol exposure led to the upregulation of Inflammation-inducing markers, including iNOS, TNF-α, COX-2, and IL-1β. In contrast, HAM co-treatment mitigated hepatocyte damage by effectively downregulating these inflammatory mediators. Collectively, these findings suggest that HAM exhibits promising hepatoprotective and anti-inflammatory properties, indicating its therapeutic potential for ALD and other inflammation-driven ailments.
酒精性肝病（ALD）是全球最常见的肝脏疾病之一，氧化应激和炎症在疾病进展中起着至关重要的作用。本研究旨在评估北美金缕梅树皮中的没食子单宁金缕梅鞣质（HAM）的抗炎特性，基于计算机对接分析预测其具有抗炎特性。为了进一步探究其作用效果，我们使用体内斑马鱼幼虫模型研究了HAM对乙醇介导的炎症的治疗作用。乙醇暴露导致肝脏炎症、氧化应激、脂质积累和肝细胞凋亡。然而，我们的研究结果表明，与HAM联合处理可显著使幼虫的抗氧化酶（如超氧化物歧化酶（SOD，35.81 U/mg蛋白质）、过氧化氢酶（CAT，33.83 μmol/mg蛋白质）和谷胱甘肽过氧化物酶（GPx，33.35 U/mg蛋白质））、一氧化氮（NO）、脂质积累、活性氧（减少19.9%）、细胞死亡（减少15.43%）、脂质过氧化（LPO，减少17.4%）和巨噬细胞浸润恢复正常。进行基因表达分析以更深入了解乙醇诱导的肝毒性以及HAM的保护作用。结果显示，乙醇暴露导致炎症诱导标志物上调，包括诱导型一氧化氮合酶（iNOS）、肿瘤坏死因子-α（TNF-α）、环氧化酶-2（COX-2）和白细胞介素-1β（IL-1β）。相比之下，HAM联合处理通过有效下调这些炎症介质减轻了肝细胞损伤。总的来说，这些发现表明HAM具有良好的肝脏保护和抗炎特性，表明其对ALD和其他炎症驱动疾病的治疗潜力。
