Wang Qiu-Hong, Lv Shao-Wa, Guo Yu-Yan, Duan Ji-Xin, Dong Shu-Yu, Wang Qiu-Shi, Yu Feng-Ming, Su Hong, Kuang Hai-Xue
Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Guangdong Pharmaceutical University, Guangzhou 510006, China.
Evid Based Complement Alternat Med. 2017;2017:8134321. doi: 10.1155/2017/8134321. Epub 2017 Dec 31.
Maxim has commonly been used as traditional Chinese medicine for the treatment of rheumatic pain and rheumatoid arthritis (RA) in China. This paper first investigated the anti-inflammation effect of extraction (CRME) on RAW264.7 cells stimulated by lipopolysaccharide (LPS) and gene expression levels of inflammatory factors. Moreover, we first evaluated the anti-RA effects of CRME using collagen-induced arthritis (CIA) in DBA/1J mice, and the incidence, clinical score, and joint histopathology were evaluated. The levels of IL-1, IL-6, TNF-, and PGE2 inflammatory factors in sera of mice were detected by enzyme-linked immunosorbent assay. The expression of NF-B p65 in the joint was tested by immune histochemical technique. The results showed that, compared with the model group, CRME significantly improved symptoms of the arthritis index, limb swelling, and histological findings by decreasing synovial membrane damage, the extent of inflammatory cell infiltration, and the expansion of capillaries in CIA mice. The results also showed that CRME can reduce the levels of IL-1, IL-6, TNF-, and PGE2 and inhibit the expression of NF-B p65. All these results indicated the anti-inflammatory efficacy of CRME as a novel botanical extraction for the treatment of RA.
在中国，威灵仙通常被用作治疗风湿疼痛和类风湿性关节炎（RA）的传统中药。本文首次研究了威灵仙提取物（CRME）对脂多糖（LPS）刺激的RAW264.7细胞的抗炎作用以及炎症因子的基因表达水平。此外，我们首次使用胶原诱导的关节炎（CIA）模型在DBA/1J小鼠中评估了CRME的抗RA作用，并对发病率、临床评分和关节组织病理学进行了评估。通过酶联免疫吸附测定法检测小鼠血清中IL-1、IL-6、TNF-α和PGE2炎症因子的水平。采用免疫组织化学技术检测关节中NF-κB p65的表达。结果表明，与模型组相比，CRME通过减少滑膜损伤、炎症细胞浸润程度和CIA小鼠毛细血管扩张，显著改善了关节炎指数、肢体肿胀和组织学表现等症状。结果还表明，CRME可以降低IL-1、IL-6、TNF-α和PGE2的水平，并抑制NF-κB p65的表达。所有这些结果表明，CRME作为一种新型植物提取物对治疗RA具有抗炎疗效。
