Wang Ping, Zhai Guanyun, Bai Yunyan
Department of Obstetrics and Gynecology, Dezhou People's Hospital, Dezhou, Shandong 253000, P.R. China.
Department of Laboratory Medicine, The Maternity and Child Health Family Planning Service Center of Decheng District, Dezhou, Shandong 253000, P.R. China.
Oncol Lett. 2018 Mar;15(3):3580-3585. doi: 10.3892/ol.2018.7791. Epub 2018 Jan 12.
The expression levels of microribonucleic acid 34a (miR-34a) and miR-218 in the serum and tumor tissues of patients with cervical cancer were investigated to explore their relationship with the diagnosis of cervical cancer. Fifty patients with cervical cancer were selected, and 30 normal patients were selected as the control group. The expression levels of miR-34a and miR-218 were detected by quantitative real-time polymerase chain reaction. The relationship between the expression of miR-34a and miR-218 was analyzed using Pearson's correlation coefficient, and the receiver operating characteristic (ROC) curve was plotted for the analysis of values of the expression of miR-34a and miR-218. The correlation of the expressions of miR-34a and miR-218 in the serum with pathological parameters and the prognosis was analyzed. The expression levels of miR-34a and miR-218 in cervical cancer patients were significantly lower than those in normal patients. The ROC curve showed the area under curve (AUC) of miR-34a was 0.893, and miR-218 was 0.794. The low expression of miR-34a in patients with cervical cancer was correlated with the degree of tumor differentiation, lymph node metastasis and the International Federation of Gynecology and Obstetrics staging. The low expression of miR-218 was related to the degree of differentiation as well as invasion and metastasis. The 5-year overall survival rate of patients was 66%, and the low expression of miR-34a and miR-218 indicated a worse survival prognosis. The low expression of miR-34a in patients with cervical cancer is related to the degree of tumor differentiation as well as invasion and metastasis, and the low expression of miR-218 is related to the degree of tumor differentiation, invasion and metastasis and clinical staging. miR-34a and miR-218 in the serum can be used as markers for the diagnosis of cervical cancer and reference indicators for the evaluation of prognosis.
研究宫颈癌患者血清和肿瘤组织中微小核糖核酸34a（miR-34a）和miR-218的表达水平，以探讨它们与宫颈癌诊断的关系。选取50例宫颈癌患者，另选30例正常患者作为对照组。采用定量实时聚合酶链反应检测miR-34a和miR-218的表达水平。利用Pearson相关系数分析miR-34a和miR-218表达之间的关系，并绘制受试者工作特征（ROC）曲线分析miR-34a和miR-218表达值。分析血清中miR-34a和miR-218表达与病理参数及预后的相关性。宫颈癌患者miR-34a和miR-218的表达水平显著低于正常患者。ROC曲线显示miR-34a的曲线下面积（AUC）为0.893，miR-218为0.794。宫颈癌患者中miR-34a低表达与肿瘤分化程度、淋巴结转移及国际妇产科联盟分期相关。miR-218低表达与分化程度以及侵袭和转移有关。患者的5年总生存率为66%，miR-34a和miR-218低表达提示生存预后较差。宫颈癌患者中miR-34a低表达与肿瘤分化程度以及侵袭和转移有关，miR-218低表达与肿瘤分化程度、侵袭和转移及临床分期有关。血清中的miR-34a和miR-218可作为宫颈癌诊断的标志物及预后评估的参考指标。
