Smith G, Skinner R P, Bliss J, Maddison P J
Clin Exp Rheumatol. 1986 Jul-Sep;4(3):209-16.
In vitro production of anti-La(SSB) and anti-DNA antibodies by mononuclear cells (mnc) from systemic lupus erythematosus (SLE) patients and healthy controls was measured using ELISA techniques. The SLE patients were divided into anti-La(SSB) positive and negative groups depending on Ouchterlony analysis of the serum. Both patient groups spontaneously produced raised levels of total IgG but not IgM compared to controls. Pokeweed mitogen (PWM) stimulated mnc from anti-La(SSB) positive SLE patients and controls gave good total IgM and IgG responses. In contrast the anti-La(SSB) negative group gave no IgG but did make an IgM response. Kinetic studies showed that 14 day cultures were essential to produce detectable amounts of specific autoantibodies which, with a few exceptions, were of IgM isotype. Low levels of anti-La(SSB) and anti-DNA were spontaneously produced by anti-La(SSB) positive SLE patients and controls. PWM stimulation at least doubled autoantibody synthesis in these two groups. The anti-La(SSB) negative SLE group gave similar responses although PWM induced autoantibody levels were reduced. ConA-induced suppressor cell assay showed that anti-La(SSB) positive SLE patients could form suppressor cells but not as effectively as controls. This group of SLE patients were more successful at suppressing IgG than IgM responses.
采用酶联免疫吸附测定（ELISA）技术，检测了系统性红斑狼疮（SLE）患者和健康对照者的单核细胞（MNC）体外产生抗La（SSB）和抗DNA抗体的情况。根据血清的双向免疫扩散分析，将SLE患者分为抗La（SSB）阳性和阴性组。与对照组相比，两组患者均自发产生了升高的总IgG水平，但IgM水平未升高。美洲商陆有丝分裂原（PWM）刺激抗La（SSB）阳性SLE患者和对照组的MNC，可产生良好的总IgM和IgG反应。相比之下，抗La（SSB）阴性组未产生IgG，但产生了IgM反应。动力学研究表明，14天的培养对于产生可检测量的特异性自身抗体至关重要，除少数例外，这些自身抗体为IgM同种型。抗La（SSB）阳性SLE患者和对照组自发产生低水平的抗La（SSB）和抗DNA。PWM刺激使这两组的自身抗体合成至少增加了一倍。抗La（SSB）阴性SLE组的反应相似，尽管PWM诱导的自身抗体水平有所降低。刀豆蛋白A诱导的抑制细胞试验表明，抗La（SSB）阳性SLE患者可形成抑制细胞，但效果不如对照组。这组SLE患者在抑制IgG反应方面比抑制IgM反应更成功。
