Gutiérrez M A, Molina J F, Jara L J, García C, Gutiérrez-Ureña S, Cuéllar M L, Gharavi A, Espinoza L R
Department of Medicine, LSU Medical Center, New Orleans, LA 70112-2822, USA.
Int Arch Allergy Immunol. 1996 Mar;109(3):229-35. doi: 10.1159/000237242.
Prolactin (PRL) has been shown to have immunoregulatory effects on a variety of immune responses. Its effect on B cell immune responses is suggested by in vitro data demonstrating a direct effect on B cell activation and differentiation, and also in vivo data demonstrating a biphasic stimulation of antibody production to sheep red blood cells. In addition, it has been shown both in animal models and patients with hyperprolactinemia that PRL may influence the presence of certain autoantibodies. The objective of this work was to study the effect of PRL on the induction of immunoglobulins, and anti-DNA and rheumatoid factor (RF) autoantibody production from peripheral blood mononuclear cells (PBMCs) from normal individuals and systemic lupus erythematosus (SLE) patients. Six female SLE patients and 10 normal individuals (5 females and 5 males) were studied. Ficoll-Hypaque-isolated PBMCs (1x10(6) cells/ml) with high concentrations of PRL (10(-4)-10(-8)M) and pokeweed mitogen (PWM) diluted to 1:400. An ELISA assay was used for immunoglobulins, RF and anti-dsDNA antibodies. PRL stimulated IgG and IgM production in a biphasic manner in normal PBMCs. Enhanced synthesis was observed at 10(-6) M, and a stimulatory effect was again observed at higher doses of PRL (10(-4))M. In contrast, only a mild stimulatory effect was observed in IgG synthesis by SLE PBMCs. These changes in Ig synthesis, however, did not reach statistical significance. PRL also induced IgG and IgM anti-dsDNA antibodies by both normal and SLE lymphocytes, but no differences were observed when compared to PWM stimulation. PRL induced IgM RF synthesis by normal lymphocytes but had no effect on SLE PBMCs. This study demonstrates that PRL induced immunoglobulin synthesis by normal and to a lesser degree by SLE lymphocytes, and also induced anti-dsDNA antibody by normal and SLE PBMCs, and IgM RF by normal PBMCs. However, the exact mechanism(s) of PRL action on the immune response awaits elucidation.
催乳素（PRL）已被证明对多种免疫反应具有免疫调节作用。体外数据表明其对B细胞活化和分化有直接影响，体内数据表明其对绵羊红细胞抗体产生有双相刺激作用，这些都提示了PRL对B细胞免疫反应的作用。此外，在动物模型和高催乳素血症患者中均已表明，PRL可能会影响某些自身抗体的存在。这项工作的目的是研究PRL对正常个体和系统性红斑狼疮（SLE）患者外周血单核细胞（PBMC）免疫球蛋白、抗DNA和类风湿因子（RF）自身抗体产生的诱导作用。研究了6名女性SLE患者和10名正常个体（5名女性和5名男性）。用Ficoll-Hypaque分离出的PBMC（1×10⁶细胞/ml），加入高浓度的PRL（10⁻⁴ - 10⁻⁸M）和稀释至1:400的商陆有丝分裂原（PWM）。采用ELISA法检测免疫球蛋白、RF和抗双链DNA抗体。PRL以双相方式刺激正常PBMC中的IgG和IgM产生。在10⁻⁶M时观察到合成增强，在更高剂量的PRL（10⁻⁴M）时再次观察到刺激作用。相比之下，SLE PBMC的IgG合成仅观察到轻微的刺激作用。然而，这些Ig合成的变化未达到统计学显著性。PRL还诱导正常和SLE淋巴细胞产生IgG和IgM抗双链DNA抗体，但与PWM刺激相比未观察到差异。PRL诱导正常淋巴细胞合成IgM RF，但对SLE PBMC无影响。这项研究表明，PRL诱导正常淋巴细胞合成免疫球蛋白，对SLE淋巴细胞的诱导程度较低，并诱导正常和SLE PBMC产生抗双链DNA抗体，以及正常PBMC产生IgM RF。然而，PRL对免疫反应作用的确切机制尚待阐明。
