Integrative Complementary Alternative Medicine Research and Development Center and Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Internal Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Biomed Res Int. 2017;2017:6135023. doi: 10.1155/2017/6135023. Epub 2017 Dec 31.
This study aimed to determine the protective effect of cashew nut-derived protein hydrolysate with high dietary fiber (AO) in cerebral ischemic rats induced by the occlusion of right middle cerebral artery (Rt.MCAO). Acute toxicity was determined and data showed that LD of AO > 5000 mg/kg BW. To determine the cerebroprotective effect of AO, male Wistar rats were orally given AO at doses of 2, 10, and 50 mg/kg for 14 days and subjected to Rt.MCAO. Brain infarction volume, neurological score, spatial memory, serum lipid profiles, and C-reactive protein together with the brain oxidative stress status were assessed. All doses of AO significantly decreased brain infarction in cortex, hippocampus, and striatum together with the decreased oxidative stress status. The improvement of spatial memory and serum C-reactive protein were also observed in MCAO rats which received AO at all doses. In addition, the decreased serum cholesterol, TG, and LDL but increased HDL were observed in MCAO rats which received high dose of AO. Taken all together, AO is the potential protectant against cerebral ischemia. The improvement of oxidative stress, inflammation, and dyslipidemia might play roles in the actions. However, further researches are required to understand the precise underlying mechanism.
本研究旨在确定腰果蛋白源膳食纤维水解物（AO）对右侧大脑中动脉闭塞（Rt.MCAO）诱导的脑缺血大鼠的保护作用。进行了急性毒性测定，数据显示 AO 的 LD > 5000mg/kgBW。为了确定 AO 的脑保护作用，雄性 Wistar 大鼠连续 14 天口服给予 2、10 和 50mg/kg 的 AO，并进行 Rt.MCAO。评估脑梗死体积、神经评分、空间记忆、血清脂质谱、C 反应蛋白以及大脑氧化应激状态。所有剂量的 AO 均显著降低大脑皮质、海马和纹状体的脑梗死面积，同时降低氧化应激状态。在接受 AO 所有剂量的 MCAO 大鼠中，还观察到空间记忆和血清 C 反应蛋白的改善。此外，在接受高剂量 AO 的 MCAO 大鼠中，观察到血清胆固醇、TG 和 LDL 降低，而 HDL 升高。综上所述，AO 是一种潜在的脑缺血保护剂。改善氧化应激、炎症和血脂异常可能在这些作用中发挥作用。然而，需要进一步的研究来了解确切的潜在机制。
