Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, Department of Biotechnology, University of Gdansk, Gdansk, Poland.
Faculty of Chemistry, Department of Analytical Chemistry and Chemical Metallurgy, Wroclaw University of Science and Technology, Wroclaw, Poland.
Biotechnol Bioeng. 2018 Jun;115(6):1581-1593. doi: 10.1002/bit.26565. Epub 2018 Mar 13.
Diseases caused by phytopathogenic bacteria are responsible for significant economic losses, and these bacteria spread through diverse pathways including waterways and industrial wastes. It is therefore of high interest to develop potent methods for their eradication. Here, antibacterial properties of direct current atmospheric pressure glow discharge (dc-APGD) generated in contact with flowing bacterial suspensions were examined against five species of phytopathogens. Complete eradication of Clavibacter michiganensis subsp. sepedonicus, Dickeya solani, and Xanthomonas campestris pv. campestris from suspensions of OD ≈ 0.1 was observed, while there was at least 3.43 logarithmic reduction in population densities of Pectobacterium atrosepticum and Pectobacterium carotovorum subsp. carotovorum. Analysis of plasma-chemical parameters of the dc-APGD system revealed its high rotational temperatures of 2,300 ± 100 K and 4,200 ± 200 K, as measured from N and OH molecular bands, respectively, electron temperature of 6,050 ± 400 K, vibrational temperature of 4000 ± 300 K, and high electron number density of 1.1 × 10 cm . In addition, plasma treatment led to formation of numerous reactive species and states in the treated liquid, including reactive nitrogen and oxygen species such as NO , NH, H O , O , O, and OH. Further examination revealed that bactericidal activity of dc-APGD was primarily due to presence of these reactive species as well as to UVA, UVB, and UVC irradiation generated by the dc-APGD source. Plasma treatment also resulted in an increase in temperature (from 24.2 to 40.2 °C) and pH (from 6.0 to 10.8) of bacterial suspensions, although these changes had minor effects on cell viability. All results suggest that the newly developed dc-APGD-based system can be successfully implemented as a simple, rapid, efficient, and cost-effective disinfection method for liquids originating from different industrial and agricultural settings.
由植物病原菌引起的疾病会造成重大的经济损失，这些细菌通过多种途径传播，包括水路和工业废物。因此，开发有效的方法来消灭它们是非常重要的。在这里，研究了与流动细菌悬浮液接触时产生的直流大气压辉光放电（dc-APGD）对五种植物病原菌的抗菌性能。从 OD≈0.1 的悬浮液中观察到完全消灭了密执安棒杆菌亚种。分离根瘤菌、野油菜黄单胞菌和野油菜黄单胞菌，而腐皮镰刀菌和胡萝卜软腐病菌亚种。胡萝卜软腐病菌的种群密度至少降低了 3.43 个对数。对 dc-APGD 系统的等离子体化学参数分析表明，其旋转温度高达 2300±100 K 和 4200±200 K，分别由 N 和 OH 分子带测量得出，电子温度为 6050±400 K，振动温度为 4000±300 K，电子数密度高 1.1×10 厘米。此外，等离子体处理导致处理液中形成了许多活性物质和状态，包括活性氮和氧物质，如 NO 、 NH 、 H O 、 O 、 O 和 OH。进一步的研究表明，dc-APGD 的杀菌活性主要归因于这些活性物质的存在以及 dc-APGD 源产生的 UVA、UVB 和 UVC 辐射。等离子体处理还导致细菌悬浮液的温度（从 24.2 到 40.2°C）和 pH 值（从 6.0 到 10.8）升高，尽管这些变化对细胞活力的影响较小。所有结果表明，新开发的基于 dc-APGD 的系统可以成功地作为一种简单、快速、高效和具有成本效益的来自不同工业和农业环境的液体消毒方法。
