Department of Analytical Chemistry and Chemical Metallurgy, Wroclaw University of Science and Technology, 27 Wybrzeze St. Wyspianskiego, 50-370 Wroclaw, Poland.
Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, University of Gdansk, 58 Abrahama, 80-307 Gdansk, Poland.
Int J Mol Sci. 2021 May 1;22(9):4813. doi: 10.3390/ijms22094813.
To the present day, no efficient plant protection method against economically important bacterial phytopathogens from the family has been implemented into agricultural practice. In this view, we have performed a multivariate optimization of the operating parameters of the reaction-discharge system, employing direct current atmospheric pressure glow discharge, generated in contact with a flowing liquid cathode (FLC-dc-APGD), for the production of a plasma-activated liquid (PAL) of defined physicochemical and anti-phytopathogenic properties. As a result, the effect of the operating parameters on the conductivity of PAL acquired under these conditions was assessed. The revealed optimal operating conditions, under which the PAL of the highest conductivity was obtained, were as follows: flow rate of the solution equaled 2.0 mL min, the discharge current was 30 mA, and the inorganic salt concentration (ammonium nitrate, NHNO) in the solution turned out to be 0.50% (/). The developed PAL exhibited bacteriostatic and bactericidal properties toward IFB0099 and IFB5103 strains, with minimal inhibitory and minimal bactericidal concentrations equaling 25%. After 24 h exposure to 25% PAL, 100% (1-2 × 10) of and cells lost viability. We attributed the antibacterial properties of PAL to the presence of deeply penetrating, reactive oxygen and nitrogen species (RONS), which were, in this case, OH, O, O, HO, HO, NH, N, N, NO, NO, and NH. Putatively, the generated low-cost, eco-friendly, easy-to-store, and transport PAL, exhibiting the required antibacterial and physicochemical properties, may find numerous applications in the plant protection sector.
迄今为止，尚未将针对来自科的经济上重要的细菌性植物病原体的有效植物保护方法应用于农业实践中。有鉴于此，我们对反应放电系统的操作参数进行了多维优化，采用与流动液体阴极（FLC-dc-APGD）接触产生的直流常压辉光放电，用于生产具有定义的物理化学和抗植物病原体特性的等离子体激活液体（PAL）。结果，评估了操作参数对在这些条件下获得的 PAL 电导率的影响。揭示了获得具有最高电导率的 PAL 的最佳操作条件如下：溶液的流速为 2.0 mL min，放电电流为 30 mA，并且溶液中的无机盐浓度（硝酸铵，NHNO）为 0.50%（/）。开发的 PAL 对 IFB0099 和 IFB5103 菌株表现出抑菌和杀菌作用，最小抑菌和最小杀菌浓度分别为 25%。经过 24 小时暴露于 25%的 PAL 后，100%（1-2×10）的和细胞失去了活力。我们将 PAL 的抗菌特性归因于具有深穿透能力的活性氧和氮物质（RONS）的存在，在这种情况下，RONS 是 OH、O、O、HO、HO、NH、N、N、NO、NO 和 NH。据称，生成的低成本、环保、易于存储和运输的 PAL 具有所需的抗菌和物理化学特性，可能在植物保护领域有多种应用。
