Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry 605006, India.
Department of Medical Gastroenterology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry 605006, India.
Clin Chim Acta. 2018 May;480:193-198. doi: 10.1016/j.cca.2018.02.017. Epub 2018 Feb 16.
BACKGROUND/AIM: It has been well established that disruption of the intestinal barrier function and increased intestinal permeability contribute to endotoxemia and associated liver injury in patients with cirrhosis. However, the relationship between systemic inflammation and tight junction protein in cirrhosis remain unidentified. The aim of this study was to assess and compare the blood concentrations of ZO-1 with systemic hsCRP in patients with cirrhosis and healthy individuals.
30 cirrhotic patients and 30 healthy individuals were enrolled in the study. Blood ZO-1 and hsCRP were measured by ELISA and biochemical parameters by AU680 Beckman Coulter (USA) autoanalyser.
The serum ALT, AST, bilirubin, gamma GT, ALP and ammonia were significantly (P < 0.0001) elevated whilst serum albumin concentration was decreased in cirrhotic patients when compared to healthy individuals. Systemic tight junction protein ZO-1 concentration [590.0 ± 32.79 vs. 349.9 ± 18.76 pg/ml, respectively; P < 0.0001] and hsCRP [10.50 ± 1.05 vs 5.31 ± 0.65 mg/L, respectively; P < 0.001] were significantly elevated in patients with cirrhosis compared to controls. Significant positive correlation was found between increased ZO-1 and hsCRP (r = 0.2680 P < 0.01).
Our results suggested that increased systemic ZO-1 concentration was associated with inflammation in cirrhosis. Thus, elevated blood ZO-1 levels could be a prognostic marker of cirrhotic patients with intestinal TJ disruption on the background of inflammation.
背景/目的：已有充分证据表明，肝硬化患者的肠道屏障功能障碍和肠道通透性增加会导致内毒素血症和相关肝损伤。然而，肝硬化患者的系统性炎症与紧密连接蛋白之间的关系尚不清楚。本研究旨在评估和比较肝硬化患者与健康个体的血液中紧密连接蛋白 ZO-1 与全身 hsCRP 的浓度。
本研究纳入了 30 例肝硬化患者和 30 例健康对照者。通过 ELISA 法测定血液 ZO-1 和 hsCRP，通过 AU680 Beckman Coulter（美国）自动分析仪测定生化参数。
与健康对照者相比，肝硬化患者的血清 ALT、AST、胆红素、γ-GT、ALP 和氨明显升高（P < 0.0001），而血清白蛋白浓度降低。与健康对照组相比，肝硬化患者的系统紧密连接蛋白 ZO-1 浓度[分别为 590.0 ± 32.79 和 349.9 ± 18.76 pg/ml；P < 0.0001]和 hsCRP[分别为 10.50 ± 1.05 和 5.31 ± 0.65 mg/L；P < 0.001]显著升高。ZO-1 与 hsCRP 之间存在显著正相关（r = 0.2680，P < 0.01）。
我们的结果表明，全身性 ZO-1 浓度的增加与肝硬化中的炎症有关。因此，血液 ZO-1 水平升高可能是肝硬化患者肠道 TJ 破坏伴炎症的预后标志物。
