Chamkasem N, Cobb W Y, Latimer G W, Salinas C, Clement B A
Robert S. Kerr Laboratories, Ada, OK 74820.
J Assoc Off Anal Chem. 1989 Mar-Apr;72(2):336-41.
A liquid chromatographic method using on-line sample cleanup, reverse flow analytical column loading, gradient elution, and postcolumn derivatization with iodine permits direct, rapid determination of aflatoxins B1, B2, G1, and G2, as well as ochratoxin A and zearalenone. Limits of quantitation are 5 ppb for the aflatoxins and ochratoxin A and 30 ppb for zearalenone. This procedure performs well as a multimycotoxin screen for cereal grains and oilseeds, with more limited success in complete animal feeds.
一种液相色谱方法，采用在线样品净化、反向流动分析柱进样、梯度洗脱以及柱后碘衍生化，可直接、快速地测定黄曲霉毒素B1、B2、G1和G2，以及赭曲霉毒素A和玉米赤霉烯酮。黄曲霉毒素和赭曲霉毒素A的定量限为5 ppb，玉米赤霉烯酮的定量限为30 ppb。该方法作为谷物和油籽的多霉菌毒素筛查方法效果良好，在全价动物饲料中的应用效果有限。
