Ko Yongseok, Yim Jeeyeo, Hwang Woon Mo, Kang Keunsoo, Ahn Tae-Young
Department of Microbiology, College of Natural Sciences, Dankook University, Cheonan 31116, Republic of Korea.
College of Biological Sciences, University of California, Davis, CA 95616, USA.
Int J Syst Evol Microbiol. 2018 Mar;68(3):782-787. doi: 10.1099/ijsem.0.002578. Epub 2018 Jan 19.
An aerobic, Gram-negative, motile by means of a single polar flagellum, and ovoid-shaped bacterium, designated D3, was isolated from shallow stream sediments in Sinan-gun, South Korea. Growth occurred at 15-40 °C (optimum 35 °C), at pH 7.0-8.0 (optimum pH 7.0), and at an optimum NaCl concentration of 0.5 % (w/v). The major cellular fatty acids (>7 % of the total) were C16 : 0, C18 : 0 2-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The predominant quinone was ubiquinone-10, and the G+C content of the genomic DNA of strain D3 was 73.1 mol%. The major polyamine was spermidine. The major polar lipids of the isolate were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D3 clustered with Roseomonas aquatica TR53 within the genus Roseomonas. The 16S rRNA gene sequence of strain D3 showed the highest sequence similarity to R. aquatica TR53 (95.9 %), followed by Roseomonas rosea 173-96 (95.7 %) and Roseomonas aerilata 5420S-30 (95.0 %). Based on the phenotypic, phylogenetic and chemotaxonomic characterization, strain D3 represents a novel species of the genus Roseomonas, for which the name Roseomonas fluminis sp. nov. is proposed. The type strain is D3 (=KACC 19269=JCM 31968).
从韩国新安郡的浅溪沉积物中分离出一株需氧、革兰氏阴性、借助单根极生鞭毛运动且呈卵形的细菌，命名为D3。该菌在15 - 40°C（最适温度35°C）、pH 7.0 - 8.0（最适pH 7.0）以及最适NaCl浓度0.5%（w/v）的条件下生长。主要细胞脂肪酸（占总量>7%）为C16 : 0、C18 : 0 2 - OH、特征性总和3（C16 : 1ω7c和/或C16 : 1ω6c）以及特征性总和8（C18 : 1ω7c和/或C18 : 1ω6c）。主要醌类为泛醌 - 10，菌株D3基因组DNA的G + C含量为73.1 mol%。主要多胺为亚精胺。该分离株的主要极性脂类为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰甘油。基于16S rRNA基因序列的系统发育分析表明，菌株D3与玫瑰单胞菌属内的水生玫瑰单胞菌TR53聚类在一起。菌株D3的16S rRNA基因序列与水生玫瑰单胞菌TR53的序列相似性最高（95.9%），其次是玫瑰色玫瑰单胞菌173 - 96（95.7%）和通气玫瑰单胞菌5420S - 30（95.0%）。基于表型、系统发育和化学分类学特征，菌株D3代表玫瑰单胞菌属的一个新物种，为此提出新名称水生玫瑰单胞菌（Roseomonas fluminis sp. nov.）。模式菌株为D3（=KACC 19269 = JCM 31968）。
