Institute of Medicinal Biotechnology, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 10050, PR China.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5711-5716. doi: 10.1099/ijsem.0.004467.
A Gram-stain-negative, aerobic, non-motile, pink-pigmented, coccus bacterium, designated CPCC 101081, was isolated from a gravel soil sample collected from Badain Jara desert, PR China. Growth of the isolate occurred at 10-37 °C and pH 5.0-8.0, with optimal growth at 28-32 °C and pH 7.0, respectively. The major cellular fatty acids were summed feature 8 (Cω7/C ω6), summed feature 3 (C ω6/Cω7) and C2-OH. Q-10 was detected as the main respiratory quinone. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipid, an amino-containing lipid and an unidentified glycophospholipid were examined in the polar lipids extraction. The 16S rRNA gene sequence comparison of strain CPCC 101081 with the available sequences in the GenBank database showed that the isolate was closely related to members of the genus , with the highest similarity to DSM 14916 (97.4 %). In the phylogenetic trees based on 16S rRNA gene sequences and the core genomes, strain CPCC 101081 was included within the clade of the genus , representing a species level, with the closest neighbor of DSM 14916 . The genomic DNA G+C content was 68.7 mol%. The average nucleotide identity and the digital DNA-DNA hybridization values between strain CPCC 101081 and the related type strains of the genus were all far lower than the cut-off values for definition species. On the basis of above phenotypic and genotypic characteristics, strain CPCC 101081 is proposed to represent a novel species of the genus with the name sp. nov. strain CPCC 101081 (=KCTC 62852=NBRC 113512) is the type strain of the species.
从中国内蒙古巴丹吉林沙漠的砾石土壤样本中分离到一株革兰氏阴性、需氧、非运动、粉红色球菌的 CPCC 101081 菌株。该分离株的生长温度为 10-37°C，pH 值为 5.0-8.0，最适生长温度分别为 28-32°C 和 pH 值为 7.0。主要细胞脂肪酸为总和特征 8（Cω7/Cω6）、总和特征 3（Cω6/Cω7）和 C2-OH。检测到 Q-10 作为主要呼吸醌。在极性脂提取物中检测到二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、一种未鉴定的磷脂、一种含氨基的脂和一种未鉴定的糖脂。通过与 GenBank 数据库中的现有序列进行比较，菌株 CPCC 101081 的 16S rRNA 基因序列与属内成员最为相似，与 DSM 14916（97.4%）最为相似。在基于 16S rRNA 基因序列和核心基因组的系统发育树中，菌株 CPCC 101081 属于属内的一个分支，代表一个种水平，与 DSM 14916 最为接近。基因组 DNA G+C 含量为 68.7 mol%。菌株 CPCC 101081 与相关属型菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值均远低于种定义的临界值。基于上述表型和基因型特征，菌株 CPCC 101081 被提议代表一个新的属种，命名为 sp. nov. 菌株 CPCC 101081（=KCTC 62852=NBRC 113512）是该种的模式菌株。
