Kuncharoen Nattakorn, Pittayakhajonwut Pattama, Tanasupawat Somboon
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand Science Park, Pathumthani 12120, Thailand.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1073-1077. doi: 10.1099/ijsem.0.002625. Epub 2018 Feb 9.
A novel endophytic actinomycete, strain WPS1-2, isolated from a root of Globba winitii C. H. Wright, was characterized taxonomically by using a polyphasic approach. Strain WPS1-2 exhibited identical characteristics to the members of the genus Micromonospora. Single spores were observed directly on substrate mycelia. The cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid. Whole-cell hydrolysates contained glucose, ribose, arabinose and xylose. The predominant menaquinones were MK-10(H8) and MK-10(H10). The major cellular fatty acids consisted of iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0. According to the 16S rRNA gene sequence of the strain, WPS1-2 showed highest similarity to Micromonospora costi CS1-12 (99.02 %). Phylogenetic analysis of the gyrase subunit B (gyrB) gene indicated that the strain was related to M. costi CS1-12. The DNA G+C content was 73.7 mol%. The strain could be distinguished from closely related type strains by using a combination of morphological, chemotaxonomic, physiological and biochemical data together with DNA-DNA relatedness values. Based on these observations, strain WPS1-2 is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora globbae sp. nov. is proposed. The type strain is WPS1-2 (=KCTC 39787=NBRC 112325=TISTR 2405).
从温氏舞花姜（Globba winitii C. H. Wright）根部分离出的一株新型内生放线菌WPS1-2，采用多相分类法对其进行了分类学特征研究。菌株WPS1-2表现出与小单孢菌属成员相同的特征。在基内菌丝体上直接观察到单个孢子。该菌株的细胞壁肽聚糖含有内消旋二氨基庚二酸和3-羟基内消旋二氨基庚二酸。全细胞水解产物含有葡萄糖、核糖、阿拉伯糖和木糖。主要的甲基萘醌是MK-10(H8)和MK-10(H10)。主要的细胞脂肪酸由异-C15 : 0、异-C16 : 0和anteiso-C15 : 0组成。根据该菌株的16S rRNA基因序列，WPS1-2与肋状小单孢菌CS1-12的相似性最高（99.02 %）。对gyrase亚基B（gyrB）基因的系统发育分析表明，该菌株与肋状小单孢菌CS1-12相关。DNA G+C含量为73.7 mol%。通过形态学、化学分类学、生理学和生化数据以及DNA-DNA相关性值的组合，该菌株可与密切相关的模式菌株区分开来。基于这些观察结果，菌株WPS1-2被认为代表小单孢菌属的一个新物种，为此提出了新名称球形小单孢菌（Micromonospora globbae sp. nov.）。模式菌株为WPS1-2（=KCTC 39787=NBRC 112325=TISTR 2405）。
