Kuncharoen Nattakorn, Kudo Takuji, Yuki Masahiro, Okuma Moriya, Pittayakhajonwut Pattama, Tanasupawat Somboon
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
Int J Syst Evol Microbiol. 2019 Sep;69(9):2884-2891. doi: 10.1099/ijsem.0.003574.
A novel endophytic actinomycete strain AZ1-13 was isolated from roots of , and its taxonomic position was investigated using a polyphasic approach. Pairwise 16S rRNA gene sequence similarities of strain AZ1-13 and its closest species, PLAI1-1 and 202201, were 99.7 and 99.2 %, respectively. Phylogenetic analyses of the family based on 16S rRNA gene sequences indicated strains AZ1-13 and PLAI1-1are located within the genus . The approximate genome size of the strain was 5.96 Mb with 71.9 mol% of G+C content. The strain AZ1-13 exhibited ANIb values of 87.4 % with PLAI1-1 and 85.1 % with 202201. Chemotaxonomic characteristics of strain AZ1-13 were consistent within the genus : cell-wall peptidoglycan of the strain contained -diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H) and MK-9(H); major cellular fatty acids were -C, 10-methyl C, C, -C and -Cω8; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids. Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13 could be distinguished from its closest neighbours, representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AZ1-13 (=KCTC 39786=NBRC 112324=JCM 32147 = TISTR 2404). This study also proposed that is transferred to the genus as comb. nov. (type strain PLAI1-1=TBRC 7644=NBRC 113144=JCM 32592).
从[植物名称]根部分离出一株新型内生放线菌菌株AZ1-13，并采用多相分类法研究其分类地位。菌株AZ1-13与其最接近的物种PLAI1-1和202201的16S rRNA基因序列两两相似性分别为99.7%和99.2%。基于16S rRNA基因序列对该科进行的系统发育分析表明，菌株AZ1-13和PLAI1-1位于[属名]属内。该菌株的基因组大小约为5.96 Mb，G+C含量为71.9 mol%。菌株AZ1-13与PLAI1-1的ANIb值为87.4%，与202201的ANIb值为85.1%。菌株AZ1-13的化学分类特征在该属内一致：该菌株细胞壁肽聚糖含有-二氨基庚二酸；葡萄糖、甘露糖、核糖和木糖为全细胞糖；主要甲基萘醌为MK-9(H)和MK-9(H)；主要细胞脂肪酸为-C、10-甲基-C、C、-C和-Cω8；检测到双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇为特征性磷脂。基于表型特征、系统发育和基因组数据，菌株AZ1-13可与其最接近的亲缘种区分开来，代表[属名]属的一个新物种，为此提出新物种名称[新物种名] sp. nov.。模式菌株为AZ1-13(=KCTC 39786=NBRC 112324=JCM 32147 = TISTR 2404)。本研究还提议将[物种名]转移至[属名]属，新组合名称为[新组合名] comb. nov.（模式菌株PLAI1-1=TBRC 7644=NBRC 113144=JCM 32592）。
