Amano Nanako, Yamamuro Ayaka, Miyahara Morio, Kouzuma Atsushi, Abe Takashi, Watanabe Kazuya
School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Graduate School of Science and Technology, Niigata University, Niigata, Niigata 950-2181, Japan.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1118-1122. doi: 10.1099/ijsem.0.002635. Epub 2018 Feb 15.
Abacterial strain, designated MMFC1, was isolated from a methanol-fed microbial fuel cell that had been inoculated with sludge obtained from a wastewater-treatmentfacility in a chemical plant. The strain grows by fermenting methanol to produce acetate under anaerobic conditions, while homoacetogenic growth is not observed. MMFC1 also grows on pyruvate and lactate but not on sugars and other organic acids. Cells are curved rods and motile, have peritrichous flagella, and form endospores. The genome sequence of strain MMFC1 supports the physiological data. Phylogenetic analysis based on the 16S rRNA gene sequence shows that strain MMFC1 is affiliated with the family Sporomusaceae, while the closest relative is Sporomusa ovata with nucleotide-sequencesimilarity of 93.5 %. Major fatty acids are iso-C13 : 0 3-OH, C16 : 1ω9 and iso-C17 : 0. On the basis of its physiological, genomic and phylogenetic features, a novel genus and species are proposed to accommodate strain MMFC1, with the name Methylomusa anaerophila gen. nov., sp. nov. The type strain of Methylomusa anaerophila is MMFC1 (=JCM 31821 = KCTC 15592).
从一个以甲醇为燃料的微生物燃料电池中分离出一株细菌，命名为MMFC1，该燃料电池接种了取自某化工厂废水处理设施的污泥。该菌株在厌氧条件下通过发酵甲醇产生乙酸盐进行生长，未观察到同型产乙酸生长。MMFC1也能利用丙酮酸和乳酸生长，但不能利用糖类和其他有机酸生长。细胞为弯曲杆菌，具运动性，有周生鞭毛，并形成芽孢。MMFC1菌株的基因组序列支持了这些生理数据。基于16S rRNA基因序列的系统发育分析表明，MMFC1菌株隶属于芽孢杆菌科，与其亲缘关系最近的是卵形芽孢杆菌，核苷酸序列相似性为93.5%。主要脂肪酸为异-C13 : 0 3-OH、C16 : 1ω9和异-C17 : 0。基于其生理、基因组和系统发育特征，提议设立一个新属和新种来容纳MMFC1菌株，命名为嗜厌氧甲基穆萨菌（Methylomusa anaerophila），新属，新种。嗜厌氧甲基穆萨菌的模式菌株为MMFC1（=JCM 31821 = KCTC 15592）。
