Schulich Faculty of Chemistry, Technion-Israel Institute of Technology, Haifa 3200008, Israel.
Schulich Faculty of Chemistry, Technion-Israel Institute of Technology, Haifa 3200008, Israel.
Curr Opin Chem Biol. 2018 Aug;45:18-26. doi: 10.1016/j.cbpa.2018.02.001. Epub 2018 Feb 20.
Histone modifications dynamically regulate chromatin structure and function, thereby mediating many processes that require access to DNA. Chemical protein synthesis has emerged as a powerful approach for generating homogeneously modified histone analogues in workable amounts for subsequent incorporation into nucleosome arrays for biochemical, functional and structural studies. This short review focuses on the strength of total chemical protein synthesis and semisynthetic approaches to generate ubiquitylated histones in their native or non-native forms and the utility of these analogues to decode the role of ubiquitylation in epigenetics.
组蛋白修饰可动态调节染色质结构和功能，从而介导许多需要 DNA 可及性的过程。化学蛋白质合成已成为一种强大的方法，可以在可行的量内生成均匀修饰的组蛋白类似物，然后将其掺入核小体阵列中，用于生化、功能和结构研究。本文简要综述了全化学蛋白质合成和半合成方法的优势，用于生成天然或非天然形式的泛素化组蛋白，以及这些类似物在解码泛素化在表观遗传学中的作用方面的应用。
