Jagiellonian University, Institute of Environmental Sciences, Gronostajowa 7, 30-387, Krakow, Poland.
Chemosphere. 2018 May;199:625-629. doi: 10.1016/j.chemosphere.2018.02.053. Epub 2018 Feb 9.
It is widely known that some toxic agents may act on DNA strand resulting in its damages. One of the possible impairments is formation of abasic sites in DNA. The aim of this study was to indicate a presence of these DNA sites in the liver tissue of bank voles inhabiting the vicinity of zinc/lead smelters. Samples that were used originated from animals collected from unpolluted (Niepołomice, Teleśnica Oszwarowa, Mikołajki) and polluted (Miasteczko Śląskie, Katowice, Olkusz) populations. They significantly differed in terms of tissue lead concentrations in the kidney and liver. The means of detected AP sites per 10 bp ranged between 3.39 (Teleśnica Oszwarowa) to 5.13 (Miasteczko Śląskie). Statistical analysis (ANOVA) showed no difference in terms of number of the AP sites between single populations. However, t-test showed significant difference between the unpolluted and polluted populations. Factorial ANOVA indicated that sex is not a factor influencing the number of AP sites. The analyses revealed statistically significant relationships between the number of AP sites and Cu concentrations in the liver, and also Pb and Cd concentrations in the kidney.
众所周知，一些有毒物质可能会作用于 DNA 链，导致其损伤。可能的损伤之一是 DNA 中形成无碱基位点。本研究的目的是表明在锌/铅冶炼厂附近栖息的田鼠肝脏组织中存在这些 DNA 位点。使用的样本来自于从未受污染（Niepołomice、Teleśnica Oszwarowa、Mikołajki）和污染地区（Miasteczko Śląskie、Katowice、Olkusz）采集的动物。这些样本在肾脏和肝脏中的组织铅浓度方面存在显著差异。每 10bp 检测到的 AP 位点数量介于 3.39（Teleśnica Oszwarowa）至 5.13（Miasteczko Śląskie）之间。统计分析（ANOVA）表明，单个种群之间的 AP 位点数量没有差异。然而，t 检验显示，未受污染和污染种群之间存在显著差异。因子方差分析表明，性别不是影响 AP 位点数量的因素。分析表明，AP 位点数量与肝脏中的铜浓度以及肾脏中的铅和镉浓度之间存在统计学上显著的关系。
