Ryu Jiin, Choi Nakyung, Kim Heejin, Kim Byung Hee, Kim Hak-Ryul, Kim In-Hwan
Department of Public Health Sciences, Graduate School, Korea University.
Department of Integrated Biomedical and Life Science, Graduate School, KOREA University.
J Oleo Sci. 2018 Mar 1;67(3):321-326. doi: 10.5650/jos.ess17198. Epub 2018 Feb 19.
Fatty acid methyl esters were synthesized from palm fatty acid distillate (PFAD) and methanol in a packed bed reactor via lipase-catalyzed esterification. The PFAD consisted of 91 wt% of free fatty acids, 2 wt% monoacylglycerides, 3 wt% diacylglycerides, and 4 wt% triacylglycerides. t-Butanol was employed as a reaction medium and a mixed enzyme consisting of Lipozyme TL IM from Thermomyces lanuginosus and Novozym 435 from Candida antarctica was employed as the biocatalyst. The effect of mixed enzyme was investigated and the optimum blending ratio (w/w) of Novozym 435 to Lipozyme TL IM was 5:95. Using the mixed enzyme, the optimum molar ratio (PFAD to methanol) and temperature were determined to be 1:6 and 30°C, respectively. Under the optimized conditions, the maximum yield of ca. 96% was achieved.
在填充床反应器中，通过脂肪酶催化酯化反应，由棕榈脂肪酸馏出物（PFAD）和甲醇合成脂肪酸甲酯。PFAD由91 wt%的游离脂肪酸、2 wt%的单酰基甘油、3 wt%的二酰基甘油和4 wt%的三酰基甘油组成。使用叔丁醇作为反应介质，并使用由来自嗜热栖热菌的Lipozyme TL IM和来自南极假丝酵母的Novozym 435组成的混合酶作为生物催化剂。研究了混合酶的效果，Novozym 435与Lipozyme TL IM的最佳混合比例（w/w）为5:95。使用该混合酶，确定最佳摩尔比（PFAD与甲醇）和温度分别为1:6和30°C。在优化条件下，实现了约96%的最大产率。
