Dos Santos Corrêa Igor Nascentes, Lorena de Souza Susana, Catran Marly, Bernardes Otávio Luiz, Portilho Márcio Figueiredo, Langone Marta Antunes Pereira
Institute of Chemistry, Rio de Janeiro State University, Rua São Francisco Xavier 524, PHLC/IQ sala 310, Rio de Janeiro, RJ 20550-013, Brazil.
Enzyme Res. 2011;2011:814507. doi: 10.4061/2011/814507. Epub 2011 May 26.
An alternative route to produce biodiesel is based on esterification of free fatty acids present in byproducts obtained from vegetable oil refining, such as palm oil fatty acid distillate (PFAD). PFAD is a byproduct of the production of edible palm oil, which contains 96 wt.% of free fatty acids. The purpose of this work was to study biodiesel synthesis via esterification of PFAD with methanol and ethanol, catalyzed by commercial immobilized lipases (Novozym 435, Lipozyme RM-IM, and Lipozyme TL-IM), in a solvent-free system. The effects of reaction parameters such as type of lipase, enzyme amount, type of alcohol, alcohol amount, and enzyme reuse were studied. Fatty acid conversion of 93% was obtained after 2.5 h of esterification reaction between PFAD and ethanol using 1.0 wt.% of Novozym 435 at 60°C.
生产生物柴油的另一种途径是基于对植物油精炼副产物中存在的游离脂肪酸进行酯化，例如棕榈油脂肪酸馏出物（PFAD）。PFAD是食用棕榈油生产过程中的一种副产物，其含有96 wt.%的游离脂肪酸。本工作的目的是研究在无溶剂体系中，通过商业固定化脂肪酶（诺维信435、Lipozyme RM-IM和Lipozyme TL-IM）催化PFAD与甲醇和乙醇进行酯化反应来合成生物柴油。研究了诸如脂肪酶类型、酶用量、醇的类型、醇用量和酶的重复使用等反应参数的影响。在60°C下，使用1.0 wt.%的诺维信435，PFAD与乙醇进行酯化反应2.5小时后，脂肪酸转化率达到了93%。
