Ultrasound-assisted enzyme extraction and properties of Shatian pomelo peel polysaccharide.

In this study, Shatian pomelo peel was used as the raw material for extracting polysaccharides using hot water extraction (HW) and ultrasonic-assisted enzyme (UVE) methods, respectively. The optimal parameters for extractingShatian pomelo peel polysaccharides (StPP) using the ultrasound-assisted enzymatic method were determined using response surface methodology (RSM). The optimal conditions for the extraction of StPP were as follows: ultrasound power 350 W, ultrasound time 50 min, enzymatic digestion time 50 min, compound enzyme addition 1.5%, and enzymatic digestion temperature 55 °C. The yield of StPP was found to be 30.1310% under these conditions. Comparing the physicochemical properties and antioxidant activity of StPP extracted using different methods, it was observed that ultrasound-assisted enzyme extraction resulted in higher yield, sugar content and glucuronic acid content of StPP compared to traditional hot water extraction. Additionally, StPP extracted by ultrasound-assisted enzyme extraction showed better antioxidant activity. These results suggest that ultrasound-assisted enzymatic extraction is an effective method to enhance the activity of natural polysaccharides.