Fujii Honoka, Horie Sachiko, Takeda Kazu, Mori Shiro, Kodama Tetsuya
Laboratory of Biomedical Engineering for Cancer, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.
Biomedical Engineering Cancer Research Center, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.
J Biophotonics. 2018 Aug;11(8):e201700401. doi: 10.1002/jbio.201700401. Epub 2018 Apr 10.
The lymphatic drug delivery system (LDDS) is a new technique that permits the injection of drugs into a sentinel lymph node (SLN) at an early stage of tumor metastasis, thereby treating metastasis in the SLN and its secondary lymph nodes (LNs). The quantity of drug required for a LDDS is much smaller than that needed for systemic chemotherapy. However, the relationship between the rate of drug injection into a SLN and the amount of drug reaching the secondary LNs has not been investigated. In this study, we used an MXH10/Mo-lpr/lpr mouse model to show that the optimal rate for the injection of a fluorescent dye by a LDDS was 10 to 80 μL/min. An injection rate of 10 to 80 μL/min was able to fill the downstream LN. However, an injection rate of 100 μL/min drove the fluorescent dye into the efferent lymphatic vessels and thoracoepigastric vein, decreasing the amount of dye retained in the downstream LN. Bolus injection (defined as an injection rate of 2400 μL/min) was unable to deliver fluorescent dye into the downstream LN. These results agree with the impulse values calculated from the injection pressures in the upstream LN. We anticipate that our findings will facilitate the development of a LDDS for use in the clinic.
淋巴药物递送系统（LDDS）是一项新技术，它能够在肿瘤转移的早期阶段将药物注射至前哨淋巴结（SLN），从而治疗SLN及其二级淋巴结（LN）中的转移灶。LDDS所需的药物量远小于全身化疗所需的量。然而，向SLN注射药物的速率与到达二级LN的药物量之间的关系尚未得到研究。在本研究中，我们使用MXH10/Mo-lpr/lpr小鼠模型表明，LDDS注射荧光染料的最佳速率为10至80μL/分钟。10至80μL/分钟的注射速率能够使下游LN充满。然而，100μL/分钟的注射速率会使荧光染料进入输出淋巴管和胸腹壁静脉，减少保留在下游LN中的染料量。推注（定义为2400μL/分钟的注射速率）无法将荧光染料输送至下游LN。这些结果与根据上游LN中的注射压力计算出的脉冲值一致。我们预计我们的研究结果将有助于开发用于临床的LDDS。
