Sukhbaatar Ariunbuyan, Kodama Tetsuya
Laboratory of Biomedical Engineering for Cancer, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Miyagi, Japan.
Department of Electronic Engineering, Graduate School of Engineering, Tohoku University, Sendai, Miyagi, Japan.
Methods Mol Biol. 2022;2524:333-346. doi: 10.1007/978-1-0716-2453-1_27.
Bioluminescence (BL) imaging is a powerful non-invasive imaging modality widely used in a broad range of biological disciplines for many types of measurements. The applications of BL imaging in biomedicine are diverse, including tracking bacterial progression, research on gene expression patterns, monitoring tumor cell growth/regression or treatment responses, determining the location and proliferation of stem cells, and so on. It is particularly valuable when studying tissues at depths of 1 to 2 cm in mouse models during preclinical research. Here we describe the protocols for the therapeutic evaluation of a lymphatic drug delivery system (LDDS) using an in vivo BL imaging system (IVIS) for the treatment of metastatic lymph nodes (LNs) with 5-fluorouracil (5-FU). The LDDS is a method that directly injects anticancer drugs into sentinel LNs (SLNs) and delivers them to their downstream LNs. In the protocol, we show that metastases in the proper axillary LN (PALN) are induced by the injection of luciferase-expressing tumor cells into the subiliac LN (SiLN) of MXH10/Mo-lpr/lpr (MXH10/Mo/lpr) mice. 5-FU is injected using the LDDS into the accessory axillary LN (AALN) to treat tumor cells in the PALN after the tumor cell growth is confirmed in the PALN. The tumor growth and therapeutic effects are evaluated by IVIS. This method can be used to evaluate tumor growth and efficacy of anticancer drugs/particles, radiotherapy, surgery, and/or a combination of these methods in various experimental procedures in the oncology field.
生物发光（BL）成像技术是一种强大的非侵入性成像方式，广泛应用于众多生物学领域的多种测量。BL成像在生物医学中的应用多种多样，包括追踪细菌进展、研究基因表达模式、监测肿瘤细胞生长/消退或治疗反应、确定干细胞的位置和增殖情况等。在临床前研究中，当在小鼠模型中研究深度为1至2厘米的组织时，它具有特别重要的价值。在此，我们描述了使用体内生物发光成像系统（IVIS）对淋巴药物递送系统（LDDS）进行治疗评估的方案，该系统用于用5-氟尿嘧啶（5-FU）治疗转移性淋巴结（LN）。LDDS是一种将抗癌药物直接注射到前哨淋巴结（SLN）并将其递送至下游淋巴结的方法。在该方案中，我们表明通过将表达荧光素酶的肿瘤细胞注射到MXH10/Mo-lpr/lpr（MXH10/Mo/lpr）小鼠的髂下淋巴结（SiLN）中，可诱导腋中淋巴结（PALN）发生转移。在确认PALN中肿瘤细胞生长后，使用LDDS将5-FU注射到腋副淋巴结（AALN）中，以治疗PALN中的肿瘤细胞。通过IVIS评估肿瘤生长和治疗效果。该方法可用于评估肿瘤生长以及抗癌药物/颗粒、放射治疗、手术和/或这些方法的组合在肿瘤学领域各种实验程序中的疗效。
