Chae K S, Yoo O J
Biochem Biophys Res Commun. 1986 Nov 14;140(3):1101-5. doi: 10.1016/0006-291x(86)90748-5.
Genes from Proteus vulgaris ATCC13315 and Brevibacterium albidum ATCC15831 were introduced into Escherichia coli, which rendered the host resistant to coliphage lambda. The clones transformed by any one of the two recombinant plasmids, pRMG101 or pRMG216, were totally resistant against the infection of virulent lambda and N4, but sensitive to ø80, T4 and T7. However, when maltose transport systems of the clones were induced by maltose, the clones were no more resistant to the phage: thus, this phenotype was thought to be due to the inhibition of phage adsorption onto the cell surface. The gene product was shown by SDS-PAGE of membrane protein-enriched extract of the clone. Molecular weight as measured was about 40,000 dalton, which coincide with that inferred from the nucleotide sequences.
将普通变形杆菌ATCC13315和白色短杆菌ATCC15831的基因导入大肠杆菌，使宿主对λ噬菌体具有抗性。用两种重组质粒pRMG101或pRMG216中的任何一种转化的克隆对毒性λ噬菌体和N4完全抗性，但对ø80、T4和T7敏感。然而，当克隆的麦芽糖转运系统被麦芽糖诱导时，这些克隆对噬菌体不再具有抗性：因此，这种表型被认为是由于噬菌体吸附到细胞表面受到抑制。通过克隆的富含膜蛋白的提取物的SDS-PAGE显示了基因产物。测得的分子量约为40,000道尔顿，与从核苷酸序列推断的分子量一致。
