Boiko V V, Romanyuk V R, Gnatyuk O P, Ilchenko O O, Karakhim S O, Korovin A V, Dovbeshko G I
Department of Physics of Biological Systems, Institute of Physics, NAS of Ukraine, Prospect Nauki, 46, Kyiv, 03680, Ukraine.
Department of Polaritonic Optoelectronics, Institute for Physics of Semiconductors, NAS of Ukraine, Prospect Nauki, 41, Kyiv, 03028, Ukraine.
J Biol Phys. 2018 Mar;44(1):101-116. doi: 10.1007/s10867-018-9480-0. Epub 2018 Feb 20.
The impact of confinement of DNA molecules in a limited volume of the cavity of photonic crystals (PC) on the vibrational properties of the DNA molecule and its conformation is studied. According to our preliminary study, the aqueous shell is removed when the DNA molecules are infiltrated into the PC cavities. Raman scattering (RS) DNA marker lines showed a dramatic conformational change of DNA in the PC cavities and the appearance of new unknown conformational states. We observed the enhancement of vibrational modes of DNA in the PC in comparison with free DNA of about tenfold and the absence of vibrational modes in DNA bases in a region of 1450-1700 cm. The observed features in the RS spectra of DNA are explained by the impact of confined interglobular volume and strong localization of the electromagnetic field. Namely, FDTD simulations in linear regime demonstrate the localization of light in cavities of PC with an approximately ninefold enhancement of the electric field within the photonic stop-band, which is the main reason for RS amplification.
研究了将DNA分子限制在光子晶体（PC）腔的有限体积内对DNA分子振动特性及其构象的影响。根据我们的初步研究，当DNA分子渗入PC腔时，水壳层会被去除。拉曼散射（RS）DNA标记线显示PC腔内DNA发生了显著的构象变化，并出现了新的未知构象状态。与游离DNA相比，我们观察到PC中DNA振动模式增强了约十倍，并且在1450 - 1700 cm区域内DNA碱基中没有振动模式。DNA的RS光谱中观察到的特征是由受限的球间体积和电磁场的强局域化作用所解释的。具体而言，线性 regime 中的FDTD模拟表明光在PC腔中局域化，在光子禁带内电场增强了约九倍，这是RS放大的主要原因。
