Aksoy Ceren, Severcan Feride
Research Coordination Department, Middle East Technical University, Ankara, Turkey.
Department of Biophysics, Altınbaş University School of Medicine, İstanbul, Turkey.
Methods Mol Biol. 2019;2045:201-215. doi: 10.1007/7651_2018_119.
The effect of aging process on stem cell function is crucial because of their critical role in tissue regeneration and repair. The impact of aging on stem cells needs to be understood clearly for the success of clinical application and obtaining desired therapeutic outcome throughout the novel stem cell based therapies. The existing methods used to monitor and characterize the stem cells have some unwanted effects on the properties of stem cells and these methods also do not provide real-time information about cellular conditions. These challenges enforce the usage of nondestructive, rapid, sensitive, high-quality, label-free, cheep, and innovative chemical monitoring methods. In this context, vibrational spectroscopy provides promising alternative to get new information into the field of stem cell biology for chemical analysis, quantification, and imaging of stem cells. Infrared spectroscopy and imaging coupled with chemometric methods can be used as novel and complimentary methods to obtain new insight into stem cell studies for future therapeutic and regenerative medicine.
衰老过程对干细胞功能的影响至关重要，因为它们在组织再生和修复中起着关键作用。为了临床应用的成功以及在整个基于新型干细胞的治疗中获得理想的治疗效果，需要清楚地了解衰老对干细胞的影响。用于监测和表征干细胞的现有方法对干细胞的特性有一些不良影响，并且这些方法也不能提供有关细胞状态的实时信息。这些挑战促使人们使用无损、快速、灵敏、高质量、无标记、廉价且创新的化学监测方法。在这种情况下，振动光谱法为在干细胞生物学领域获取有关干细胞化学分析、定量和成像的新信息提供了有前景的替代方法。红外光谱和成像结合化学计量学方法可以用作新颖且互补的方法，以在未来的治疗和再生医学中获得对干细胞研究的新见解。
