Department of Pharmaceutical Chemistry, School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia.
Department of Human Biology, School of Medicine, International Medical University, Kuala Lumpur, Malaysia.
Phytother Res. 2018 Jun;32(6):1108-1118. doi: 10.1002/ptr.6051. Epub 2018 Feb 21.
In this study, a series of 20 structurally similar vanilloids (Vn) were tested for their antiproliferative effects against 12 human cancer cells: human breast (MCF-7 and MDA-MB-231), cervical (HeLa), ovarian (Caov-3), lung (A549), liver (HepG2), colorectal (HT-29 and HCT116), nasopharyngeal (CNE-1 and HK-1), and leukemic (K562 and CEM-SS) cancer cells. Among all the tested vanilloids, Vn16 (6-shogaol) exhibited the most potent cytotoxic effects against human colorectal cancer cells (HT-29). The apoptotic induction effects exhibited by Vn16 on HT-29 cells were confirmed using dual staining fluorescence microscopy and enzyme-linked immunosorbent assay. The effects of Vn16 on regulation of 43 apoptotic-related markers were determined in HT-29. The results suggested that 8 apoptotic markers (caspase 8, BAD, BAX, second mitochondrial-derived activator, caspase 3, survivin, bcl-2, and cIAP-2) were either upregulated or downregulated. These results further support the chemopreventive properties of foods that contain vanilloids.
在这项研究中，测试了一系列 20 种结构相似的香草素（Vn）对 12 种人类癌细胞的抗增殖作用：人乳腺癌（MCF-7 和 MDA-MB-231）、宫颈癌（HeLa）、卵巢癌（Caov-3）、肺癌（A549）、肝癌（HepG2）、结直肠癌（HT-29 和 HCT116）、鼻咽癌（CNE-1 和 HK-1）和白血病（K562 和 CEM-SS）。在所有测试的香草素中，Vn16（6-姜烯酚）对人结直肠癌细胞（HT-29）表现出最强的细胞毒性作用。通过双染色荧光显微镜和酶联免疫吸附试验证实了 Vn16 对 HT-29 细胞的凋亡诱导作用。在 HT-29 中测定了 Vn16 对调节 43 个凋亡相关标记物的影响。结果表明，8 个凋亡标记物（半胱天冬酶 8、BAD、BAX、线粒体衍生的第二激活物、半胱天冬酶 3、存活素、bcl-2 和 cIAP-2）被上调或下调。这些结果进一步支持了含有香草素的食物的化学预防特性。
