Priyanka B S, Rastogi Navin K
Dept. of Food Engineering, Academy of Scientific and Innovative Research, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, 570020, India.
Biotechnol Prog. 2018 May;34(3):721-729. doi: 10.1002/btpr.2624. Epub 2018 Mar 6.
This work deals with the extraction of lipase and amylase from enzyme mixture by employing liquid emulsion membranes (LEM). The electrostatic interaction between enzymes and reverse micellar surfactant polar head group plays an important role for selective extraction of two different enzymes having different isoelectric points. The optimized conditions for lipase extraction (pH 7.0) resulted in the purification fold and activity recovery of 5.43 fold and 89.53%, respectively, whereas, in case of amylase (pH 9.0) the purification fold and activity recovery were 6.58 and 94.32%, respectively. The results were compared with the control sample (containing individual enzymes) and mixture of enzymes lipase and amylase and it was shown that for optimum conditions the activity recovery and purification fold was higher for the individual enzymes as compared to their mixture. Downstream processing involving LEM was shown to be a feasible method for selective extraction of enzymes. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:721-729, 2018.
本研究通过采用液膜乳液（LEM）从酶混合物中提取脂肪酶和淀粉酶。酶与反胶束表面活性剂极性头基之间的静电相互作用对于选择性提取具有不同等电点的两种不同酶起着重要作用。脂肪酶提取的优化条件（pH 7.0）导致纯化倍数和活性回收率分别为5.43倍和89.53%，而对于淀粉酶（pH 9.0），纯化倍数和活性回收率分别为6.58和94.32%。将结果与对照样品（含单一酶）以及脂肪酶和淀粉酶的酶混合物进行比较，结果表明，在最佳条件下，单一酶的活性回收率和纯化倍数高于其混合物。结果表明，涉及液膜乳液的下游加工是一种选择性提取酶的可行方法。© 2018美国化学工程师学会生物技术进展，34:721 - 729，2018年。
