采用三种等电聚焦方法对对照血痕和案件血痕中血型特异性成分（Gc）和磷酸葡萄糖变位酶（PGM1）位点分型的敏感性比较。
A comparison of the sensitivity of typing group-specific component (Gc) and the phosphoglucomutase (PGM1) locus in control and casework bloodstains by three isoelectric focusing methods.
作者信息
Nelson L, Westwood S A, Werrett D J
出版信息
Forensic Sci Int. 1986 Oct;32(2):121-30. doi: 10.1016/0379-0738(86)90148-9.
The performance of typing group-specific component (Gc) in bloodstains by two isoelectric focusing methods followed by its detection with silver staining has been compared with an established forensic system of typing phosphoglucomutase (PGM1) locus phenotypes by isoelectric focusing (IEF) in 1 mm gels. For Gc typing ultra-thin isoelectric focusing (UTIEF) gels and immobilized pH gradient (IPG) gels were used. Both laboratory prepared stains and casework stains were examined. The Gc UTIEF method is approximately eight times more sensitive than the existing PGM1 1 mm IEF method for control and casework stains. However, on average, a larger amount of stain was taken from casework stains than control stains for each typing system. A total of 53 casework stains were examined. Comparable success rates of 62% and 64% were obtained for typing Gc on UTIEF gels and PGM1 by 1 mm IEF, respectively. A success rate of 55% was obtained for typing Gc on IPGs. Bloodstains that were over 200 days old were successfully grouped by all three methods.
通过两种等电聚焦方法对血迹中的血型特异性成分（Gc）进行分型，随后用银染法进行检测，并与一种已确立的法医系统进行了比较，该系统通过在1毫米凝胶中进行等电聚焦（IEF）来分型磷酸葡萄糖变位酶（PGM1）位点的表型。对于Gc分型，使用了超薄等电聚焦（UTIEF）凝胶和固定化pH梯度（IPG）凝胶。对实验室制备的污渍和实际案件中的污渍都进行了检测。对于对照污渍和实际案件污渍，Gc UTIEF方法的灵敏度大约是现有的PGM1 1毫米IEF方法的八倍。然而，平均而言，对于每个分型系统，从实际案件污渍中取用的污渍量比对照污渍要多。总共检测了53个实际案件污渍。在UTIEF凝胶上对Gc进行分型和通过1毫米IEF对PGM1进行分型，分别获得了62%和64%的可比成功率。在IPG上对Gc进行分型的成功率为55%。所有这三种方法都成功地对超过200天的陈旧血迹进行了分型。
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