Muñoz-Barús I, Carracedo A, Pascali V L
Instituto de Medicina Legal, Santiago de Compostela, Spain.
Electrophoresis. 1992 Apr;13(4):239-43. doi: 10.1002/elps.1150130149.
Some polymorphic proteins (alpha 1-antitrypsin, orosomucoid, transferrin, group specific component, plasminogen) and enzymes (phosphoglucomutase, acid phosphatase, estrase D) were determined in bloodstain extracts by isoelectric focusing with carrier ampholytes (CA) and with immobilized pH gradients (IPGs) rehydrated with CA. IPGs yield superior results for typing of genetics markers in bloodstains since phenotypes are better distinguished and the bands are straighter and sharper in the presence of contaminants. Also, the sensitivity of IPGs with CA is similar to isoelectric focusing (IEF) with CA. A new variant, ACPB1, found in Negroid west African populations and not found in Caucasians is described. Such a variant can only be determined by IPGs since its isoelectric point (pI 5.95) is close to that of the ACPB (pI 6.05) variant.
通过使用载体两性电解质（CA）以及用CA复水的固定化pH梯度（IPG）进行等电聚焦，测定了血迹提取物中的一些多态性蛋白质（α1 -抗胰蛋白酶、类粘蛋白、转铁蛋白、群体特异性成分、纤溶酶原）和酶（磷酸葡萄糖变位酶、酸性磷酸酶、酯酶D）。由于在存在污染物的情况下，表型能得到更好的区分，条带更直且更清晰，所以IPG在血迹遗传标记分型方面产生了更优的结果。此外，带有CA的IPG的灵敏度与带有CA的等电聚焦（IEF）相似。本文描述了一种在西非黑人人群中发现而在高加索人群中未发现的新变体ACPB1。由于其等电点（pI 5.95）与ACPB变体（pI 6.05）接近，所以这种变体只能通过IPG来测定。
