Muñoz-Barús I, Lareu M V, López-Rodriguez I, Rodriguez-Calvo M S, Carracedo A
Department of Legal Medicine, Faculty of Medicine, University of Santiago, La Coruña, Spain.
Z Rechtsmed. 1989;102(4):271-5. doi: 10.1007/BF00201323.
PGM1, ESD, and ACP were determined in bloodstain extracts by isoelectric focusing (IEF) with carrier ampholytes (CA) and HIEF. HIEF yields superior results in PGM typing from bloodstain extracts, whereas for ESD and ACP typing isoelectric focusing with carrier ampholytes seems to be the method of choice.
通过使用载体两性电解质(CA)的等电聚焦(IEF)和高压等电聚焦(HIEF),对血迹提取物中的磷酸葡萄糖变位酶1(PGM1)、酯酶D(ESD)和酸性磷酸酶(ACP)进行了测定。在对血迹提取物进行PGM分型时,高压等电聚焦产生了更好的结果,而对于ESD和ACP分型,使用载体两性电解质的等电聚焦似乎是首选方法。