Department of Internal Medicine, Nowon Eulji Medical Center, Eulji University School of Medicine, Seoul, Korea.
Department of Internal Medicine, Hanyang University School of Medicine, Seoul, Korea.
Korean J Intern Med. 2019 Jul;34(4):777-784. doi: 10.3904/kjim.2017.100. Epub 2018 Feb 23.
BACKGROUND/AIMS: Intestinal cholesterol absorption includes intestinal Niemann-Pick C1-like 1 (NPC1L1) and is an important target pathway in nonalcoholic fatty liver disease (NAFLD). We investigated the expression of NPC1L1 and its correlation with liver X receptor (LXR) expression in peripheral mononuclear (PMN) cells in patients with NAFLD.
We evaluated intestinal expression of NPC1L1 in 25 NAFLD patients and 28 healthy controls. We calculated the mRNA expression levels of LXR and farnesoid X receptor (FXR), which are master players of cholesterol metabolism in PMN cells. The protein expression of ABCA1, ABCG5/8, NPC1L1, SREBP, LXR, FXR, and CD36 was measured on tissue samples from the duodenum and ileum.
The expression of LXR (p = 0.01) and FXR (p = 0.03) in PMN cells was increased in the NAFLD group compared to the control group. Duodenal NPC1L1 decreased in the NAFLD group compared to the healthy controls (3.38 ± 1.4 vs. 2.42 ± 1.2, p = 0.05). NPC1L1 expression in the duodenum was negatively correlated with LXR expression in PMN cells. Expression of LXR and FXR in the ileum was also negatively correlated with the expression of LXR in PMN cells.
Duodenal NPC1L1 expression was decreased in NAFLD and was negatively correlated with LXR expression in PMN cells.
背景/目的：肠道胆固醇吸收包括肠道 Niemann-Pick C1 样 1（NPC1L1），是非酒精性脂肪性肝病（NAFLD）的重要靶点途径。我们研究了 NPC1L1 在 NAFLD 患者外周血单核细胞（PMN）中的表达及其与肝 X 受体（LXR）表达的相关性。
我们评估了 25 例 NAFLD 患者和 28 例健康对照者的肠道 NPC1L1 表达。我们计算了胆固醇代谢的主调控因子 LXR 和法尼醇 X 受体（FXR）在 PMN 细胞中的 mRNA 表达水平。在十二指肠和回肠组织样本上测量了 ABCA1、ABCG5/8、NPC1L1、SREBP、LXR、FXR 和 CD36 的蛋白表达。
与对照组相比，NAFLD 组 PMN 细胞中 LXR（p = 0.01）和 FXR（p = 0.03）的表达增加。与健康对照组相比，NAFLD 组十二指肠 NPC1L1 表达降低（3.38 ± 1.4 对 2.42 ± 1.2，p = 0.05）。十二指肠 NPC1L1 表达与 PMN 细胞中 LXR 表达呈负相关。回肠中 LXR 和 FXR 的表达也与 PMN 细胞中 LXR 的表达呈负相关。
NAFLD 患者十二指肠 NPC1L1 表达降低，与 PMN 细胞中 LXR 表达呈负相关。
