Department of Electrical Engineering and Computer Science, Cleveland State University, Cleveland, Ohio, USA.
Department of Emergency Medicine, University of Michigan, Ann Arbor, Michigan, USA.
Sci Rep. 2018 Feb 21;8(1):3416. doi: 10.1038/s41598-018-21520-9.
The current culture-based approach for the diagnosis of bloodstreams infection is incommensurate with timely treatment and curbing the prevalence of multi-drug resistant organisms (MDROs) due to its long time-to-result. Bloodstream infections typically involve extremely low (e.g., <10 colony-forming unit (CFU)/mL) bacterial concentrations that require a labor-intensive process and as much as 72 hours to yield a diagnosis. Here, we demonstrate a culture-free approach to achieve rapid diagnosis of bloodstream infections. An immuno-detection platform with intrinsic signal current amplification was developed for the ultrasensitive, rapid detection, identification (ID) and antibiotic susceptibility testing (AST) of infections. With its capability of monitoring short-term (1-2 hours) bacterial growth in blood, the platform is able to provide 84-minute simultaneous detection and ID in blood samples below the 10 CFU/mL level and 204-minute AST. The susceptible-intermediate-resistant AST capacity was demonstrated.
目前基于培养的血液感染诊断方法由于其结果耗时较长，因此无法及时进行治疗和遏制多药耐药菌（MDROs）的流行。血流感染通常涉及极低的细菌浓度（例如，<10 个集落形成单位（CFU）/毫升），这需要一个劳动密集型的过程，并且需要长达 72 小时才能得出诊断。在这里，我们展示了一种无需培养的方法来实现血流感染的快速诊断。开发了一种具有固有信号电流放大功能的免疫检测平台，用于对感染进行超灵敏、快速的检测、鉴定（ID）和抗生素药敏试验（AST）。该平台能够监测血液中的短期（1-2 小时）细菌生长，能够在低于 10 CFU/mL 水平的血液样本中提供 84 分钟的同步检测和 ID，以及 204 分钟的 AST。证明了敏感-中介-耐药 AST 能力。
