Holda J H, Maier T, Claman H N
J Immunol. 1986 Dec 1;137(11):3538-43.
Natural suppressor (NS) cells isolated from the spleens of mice 34 or more days after induction of chronic graft-vs-host disease showed potent suppressor activity that was dependent on the presence of lymphokines such as those found in lectin-free Con A supernatant (CAS). Both IL 2 and IFN-gamma were found to enhance NS activity, with IFN-gamma being the most active molecule. To generalize these findings, normal adult bone marrow (BM), a second environment where NS activity has been reported, was examined. Normal adult BM cells nonspecifically suppressed both B and T cell mitogen responses. BM suppression could also be enhanced by the addition of CAS to the cultures. Removal of plastic-adherent cells, Thy-1.2-positive cells, or B cells did not significantly diminish suppression or remove the ability to enhance suppression with CAS. When isolated on discontinuous Percoll gradients, BM suppressor cells banded in the less dense fractions, and the suppression in these bands was also enhanced by lymphokines. The suppressive activity of these cells was greatly enhanced by the addition of CAS, IFN-gamma, or IL 2. However, IFN-gamma appears to be a more potent enhancing molecule, being active at levels between 1 and 10 U/ml.
在诱导慢性移植物抗宿主病34天或更长时间后从小鼠脾脏中分离出的天然抑制细胞（NS）表现出强大的抑制活性，这种活性依赖于淋巴细胞因子的存在，如在无凝集素的刀豆蛋白A上清液（CAS）中发现的那些因子。白细胞介素2（IL 2）和γ干扰素（IFN-γ）都被发现可增强NS活性，其中IFN-γ是最具活性的分子。为了推广这些发现，对正常成年骨髓（BM）进行了检查，正常成年骨髓是另一个已报道有NS活性的环境。正常成年骨髓细胞非特异性地抑制B细胞和T细胞有丝分裂原反应。向培养物中添加CAS也可增强骨髓抑制作用。去除贴壁细胞、Thy-1.2阳性细胞或B细胞并不会显著降低抑制作用，也不会消除用CAS增强抑制作用的能力。当在不连续的Percoll梯度上分离时，骨髓抑制细胞聚集在密度较低的组分中，这些组分中的抑制作用也会被淋巴细胞因子增强。通过添加CAS、IFN-γ或IL 2，这些细胞的抑制活性会大大增强。然而，IFN-γ似乎是一种更有效的增强分子，在1至10 U/ml的水平上具有活性。
